Dear Teresa,

Because we also wanted to place cover slips with a different thickness, 
we decided to buy the FocalCheck solution. In principal this should work 
in your case, as well. You can just dry them on the slide together with 
the cover slip. Unfortunatly, in this configuration they don't last a 
long time. Some of the FocalChecks got destroyed, when I tried to mount 
them for a longer time. This was the case when I first dried them (~ 
5µl) for ~ 1 h on the coverslip and than added mounting medium (dako, 
fluorescence mounting media). The drying time is then critical. I didn't 
like the fluorescence mounting media of molecular probes, because the 
probes haven't been strongly fixed, but maybe it works better with the 
FocalChecks.

cheers,

Katharina



MICROSCOPIA CONFOCAL y CCD schrieb:
> Does anybody have experience with the FocalCheck Fluorescence 
> Microscope Test Slides from Molecular Probes.?
> I bought Test Slide #1 and I'd focus problems especially with oil 
> immersion objectives in different microscopes. They have sent me 
> another one and I'm having the same trouble focusing.I do not 
> understand what is happening. ¿Someone has had problems with this kit?
>
> Thanks in advance
>
> M. Teresa
>
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