YFP-mOrange should work for you
We are using GFP-mOrange as FRET pair. You should have a better quantum yield for the donor compared to acceptor. 
Ammasi


-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Maria Calvo
Sent: Friday, March 26, 2010 11:48 AM
To: [log in to unmask]
Subject: Best FRET pair

Dear all,

We are planning to do FRET experiments with a fluorescent protein (YFP, mCherry or mOrange)and a directly labelled primary antibody. 

We are restricted to YFP, mCherry or mOrange for one of the proteins (the most abundant). 

Does anyone know what's the best FRET pair for any of these FPs, in terms of J (overlap), brightness ?

Thanks for your help,

Maria Calvo

-- 
Ammasi Periasamy, Ph.D.
Director, Keck Center for Cellular Imaging (KCCI)
Professor of Biology and Biomedical Engineering
Biology, Gilmer Hall (064), 485 McCormick Rd
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Voice: 434-243-7602 (Office); 982-4869 (lab)
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10th Annual Workshop on FRET Microscopy, March 8-12, 2011
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