We do the same thing as Katrina mentioned. We bought Focal Check as well as the sub-resolution beads for finding out PSF and made and our own slides. The focal check and the subresolution beads come with protocols for making slides (http://probes.invitrogen.com/media/pis/mp07234.pdf ). You need a very little quantity to make slides, I dilute the beads before spotting them on the coverglass, I prepared my slides as per the protocol and they lasted for a long time. 

No commercial interest! 


Best,

Neeraj. 

Neeraj V. Gohad, Ph.D.
Postdoctoral Fellow
Okeanos Research Group
Department of Biological Sciences
132 Long Hall
Clemson University 
Clemson,SC-29634
Phone: 864-656-3597
Fax: 864-656-0435

Website: http://www.clemson.edu/okeanos  

Please note my new email address: [log in to unmask]



-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Katharina Thomsen
Sent: Friday, March 26, 2010 12:18 PM
To: [log in to unmask]
Subject: Re: FocalCheck test slide

Dear Teresa,

Because we also wanted to place cover slips with a different thickness,
we decided to buy the FocalCheck solution. In principal this should work
in your case, as well. You can just dry them on the slide together with
the cover slip. Unfortunatly, in this configuration they don't last a
long time. Some of the FocalChecks got destroyed, when I tried to mount
them for a longer time. This was the case when I first dried them (~
5µl) for ~ 1 h on the coverslip and than added mounting medium (dako,
fluorescence mounting media). The drying time is then critical. I didn't
like the fluorescence mounting media of molecular probes, because the
probes haven't been strongly fixed, but maybe it works better with the
FocalChecks.

cheers,

Katharina



MICROSCOPIA CONFOCAL y CCD schrieb:
> Does anybody have experience with the FocalCheck Fluorescence
> Microscope Test Slides from Molecular Probes.?
> I bought Test Slide #1 and I'd focus problems especially with oil
> immersion objectives in different microscopes. They have sent me
> another one and I'm having the same trouble focusing.I do not
> understand what is happening. ¿Someone has had problems with this kit?
>
> Thanks in advance
>
> M. Teresa
>
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