It is much easier to find the surface on which the subresolution beads are mounted if a few larger beads of 6 um  or 15 um beads are added to the diluted suspension.  

Regards,
Glen

Glen MacDonald
Core for Communication Research
Virginia Merrill Bloedel Hearing Research Center
Box 357923
University of Washington
Seattle, WA 98195-7923  USA
(206) 616-4156
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On Mar 26, 2010, at 11:05 AM, Neeraj Gohad wrote:

> We do the same thing as Katrina mentioned. We bought Focal Check as well as the sub-resolution beads for finding out PSF and made and our own slides. The focal check and the subresolution beads come with protocols for making slides (http://probes.invitrogen.com/media/pis/mp07234.pdf ). You need a very little quantity to make slides, I dilute the beads before spotting them on the coverglass, I prepared my slides as per the protocol and they lasted for a long time. 
> 
> No commercial interest! 
> 
> 
> Best,
> 
> Neeraj. 
> 
> Neeraj V. Gohad, Ph.D.
> Postdoctoral Fellow
> Okeanos Research Group
> Department of Biological Sciences
> 132 Long Hall
> Clemson University 
> Clemson,SC-29634
> Phone: 864-656-3597
> Fax: 864-656-0435
> 
> Website: http://www.clemson.edu/okeanos  
> 
> Please note my new email address: [log in to unmask]
> 
> 
> 
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Katharina Thomsen
> Sent: Friday, March 26, 2010 12:18 PM
> To: [log in to unmask]
> Subject: Re: FocalCheck test slide
> 
> Dear Teresa,
> 
> Because we also wanted to place cover slips with a different thickness,
> we decided to buy the FocalCheck solution. In principal this should work
> in your case, as well. You can just dry them on the slide together with
> the cover slip. Unfortunatly, in this configuration they don't last a
> long time. Some of the FocalChecks got destroyed, when I tried to mount
> them for a longer time. This was the case when I first dried them (~
> 5µl) for ~ 1 h on the coverslip and than added mounting medium (dako,
> fluorescence mounting media). The drying time is then critical. I didn't
> like the fluorescence mounting media of molecular probes, because the
> probes haven't been strongly fixed, but maybe it works better with the
> FocalChecks.
> 
> cheers,
> 
> Katharina
> 
> 
> 
> MICROSCOPIA CONFOCAL y CCD schrieb:
>> Does anybody have experience with the FocalCheck Fluorescence
>> Microscope Test Slides from Molecular Probes.?
>> I bought Test Slide #1 and I'd focus problems especially with oil
>> immersion objectives in different microscopes. They have sent me
>> another one and I'm having the same trouble focusing.I do not
>> understand what is happening. ¿Someone has had problems with this kit?
>> 
>> Thanks in advance
>> 
>> M. Teresa
>> 
>> SERVICIO de MICROSCOPIA CONFOCAL y CCD
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>> Oscar Hidalgo Blanco
>> Amadeo Cazaña Soto
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