Hi Jacqui,
     I list the problems on our EMCCD here:
     1)	The brightness of images field is not equal. The left-bottom corner
is much dimmer than other fields.
3)	The EMCCD’s sensitivities are much lower than before. Even for strong
fluorescence signal, the images look quite dim. It is not a single-photon
CCD any longer.
4)	When set the EM gain’s value at 4.7, the background image is not uniform. 
5)	There seems something wrong with the output TTL trigger signal’s voltage
value. We use the “fire” output to trigger the shutter’s open/close.
Recently three shutter drivers from the uniblitz are broken down during a
week. We guess the trigger signal from the EMCCD is not stable.
          Abover are the problems I can find.
  About the cooling system of the EMCCD, it seems ok. The cooling
temperature looks normal, at least show normal by the control software.  If
there are seal problems as Jim mentioned, what can I do?
           I appreciated your reply very much. I attend a meeting during the
past days, so sorry for my delayed reply.
         Zhan Cheng
     
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