Hi-

We have a collaborator who is interested in confocal microscopy of C. 
elegans. We have not done work with this organism and are looking for the 
best way to immobilize the worm and prepare the sample for imaging on our 
spinning disk confocal microscope. We have a typical inverted microscope set-
up (Nikon body). 
The collaborator prefers to keep the worm(s) alive, so we need a method to 
immobilize them (anesthesia?).
Do folks use agar when mounting samples? Which slides are typically used for 
this purpose? If there is a reference, I would love to get the citation.

Thanks,
Jay Vyas
Massachusetts General Hospital