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Hello everybody.  This isn't exactly a microscopy question, but maybe someone here has experience with this issue.

We are doing experiments involving assessing the viability of cells in whole mount live organs (liver, in this case), and we are using Mitotracker in order to see whether the cells are alive.  Lately we have been doubting the integrity of our Mitotracker staining, and we would like a negative control to work with.  Does anybody have any experience with stopping the mitochondrial activity in live tissues, like anything that we could inject into the organ?  We need these negative controls to be on tissue, not on cells.  Any advice you can give will be greatly appreciated.

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Yevgeniy Romin

Digital Microscopist
Memorial Sloan-Kettering Cancer Center
Molecular Cytology Core Facility
1275 York Ave. Box 333
New York, NY 10065
Tel.646-888-2186
Fax. 646-422-0640
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