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Hello everybody

Lately I've been getting mixed responses to the following question - How much can one trust FRET measurements in the cell membrane?  There are many papers published showing FRET in the membrane (proteins involved in cell movement, actin polymerization, etc.)  But I've also heard statements made that because of the dynamics in the cell membrane there is great possibility of random FRET occurring and the FRET data acquired from cell membranes is quite often artifactual.  Has anybody had any experience with this issue before?

Thanks in advance for your always helpful responses,

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Yevgeniy Romin

Digital Microscopist
Memorial Sloan-Kettering Cancer Center
Molecular Cytology Core Facility
1275 York Ave. Box 333
New York, NY 10065
Tel.646-888-2186
Fax. 646-422-0640
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