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Dear Dr. Spenser--
On 1/27/2011 7:19 PM, Kathryn Spencer wrote:
> I'm in a quandary. We are looking at getting an additional confocal system in our core (we have the FV500). We're looking at either the Olympus FV1000 or the Nikon C2. Both companies have been very, very aggressive in pricing and components, to where our two quotes are essentially identical. Same lasers, same PMTs, same multi-dimensional acquisition.
> This system would be used 95% for fixed tissue imaging, some cells. Moderately to highly sophisticated users. No real FRAPing or uncaging, or other modalities than simple, multi-color fluorescence Z-stacks.
> Service from both companies is exemplary, and has been over the years. We are so fortunate to have other additional systems to meet other imaging needs, so I am not concerned about expandability, or future capabilities.
> What a fabulous quandary to have. Which system is better? I've talked to users on both sides, who are completely satisfied with their choice.
> Recommendations? Which one out-performs the other? Honestly, that will be the difference. Which has better signal to noise? Which has faster scans?
> Thanks.
Given your use for the instrument (--i.e. multicolor z-stacks) and the
recent discussions on this list about chromatic aberration, I would
suggest testing for chromatic correction before buying. That could be
done either with tetraspec beads, or by doing a 3- (or 4-) color
reflectance scan off a mirror. In either case you'll want to collect
your images using simultaneous (not sequential) scanning.
If you do this, let us know what you see!
Martin
--
Martin Wessendorf, Ph.D. office: (612) 626-0145
Assoc Prof, Dept Neuroscience lab: (612) 624-2991
University of Minnesota Preferred FAX: (612) 624-8118
6-145 Jackson Hall, 321 Church St. SE Dept Fax: (612) 626-5009
Minneapolis, MN 55455 e-mail: [log in to unmask]
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