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Dear Confocal listserv,

Who has purchased a fluorescence nanoscope? What can you tell me about 
your experiences - either here or privately?

I see 11 OMX labs listed at http://www.api.com/omx-labs.asp (may be out 
of date or some customers may be shy).

I am aware of STED systems in the USA at Yale Univ, NIH and I was told 
San Diego (have not found where), and possibly UC Denver. Paul French 
apparently did his own upgrade of a Leica SP2 
(http://www.focusonmicroscopy.org/2008/PDF/070_Auksorius.pdf and a Leica 
Scientific Forum video ... see also related work at 
ftp://ftp.espci.fr/incoming/Gilles/INSERM-STED-x6.pdf ).

I am also aware of one 4pi microscope in the USA.

So, who has N-SIM, N-STORM, SR-SIM, PALm, SR-200, and what can you tell 
me about your experiences with your (purchased) nanoscope(s)?

Sincerely,

George
p.s. Please no need to clutter up the listserv with other people's 
nanoscope references - I know how to use PubMed. For that matter, I've 
replicated the results of the following two papers on my confocal's:

    Subdiffraction fluorescence imaging of biomolecular structure and
    distributions with quantum dots. </pubmed/20600360>

    Heidbreder M, Endesfelder U, van de Linde S, Hennig S, Widera D,
    Kaltschmidt B, Kaltschmidt C, Heilemann M.

    Biochim Biophys Acta. 2010 Oct;1803(10):1224-9. Epub 2010 Jun
    23.PMID: 20600360


    Quantum dot triexciton imaging with three-dimensional subdiffraction
    resolution. </pubmed/19453186>

    Hennig S, van de Linde S, Heilemann M, Sauer M.

    Nano Lett. 2009 Jun;9(6):2466-70.PMID: 19453186

I have used <1 Airy unit pinhole to improve triexciton resolution 
further. I have not tried doing 3D deconvolution on the data.


-- 


George McNamara, PhD
Analytical Imaging Core Facility
University of Miami