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UCLA has a STED

http://clms.cnsi.ucla.edu/cnsi/clms/equipment-list?search_lab=6791
<http://clms.cnsi.ucla.edu/cnsi/clms/equipment-list?search_lab=6791>
http://www.cnsi.ucla.edu/staticpages/core-facilities#alms


<http://www.cnsi.ucla.edu/staticpages/core-facilities#alms>

On Sat, Jan 29, 2011 at 7:32 AM, George McNamara
<[log in to unmask]>wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Dear Confocal listserv,
>
> Who has purchased a fluorescence nanoscope? What can you tell me about your
> experiences - either here or privately?
>
> I see 11 OMX labs listed at http://www.api.com/omx-labs.asp (may be out of
> date or some customers may be shy).
>
> I am aware of STED systems in the USA at Yale Univ, NIH and I was told San
> Diego (have not found where), and possibly UC Denver. Paul French apparently
> did his own upgrade of a Leica SP2 (
> http://www.focusonmicroscopy.org/2008/PDF/070_Auksorius.pdf and a Leica
> Scientific Forum video ... see also related work at
> ftp://ftp.espci.fr/incoming/Gilles/INSERM-STED-x6.pdf ).
>
> I am also aware of one 4pi microscope in the USA.
>
> So, who has N-SIM, N-STORM, SR-SIM, PALm, SR-200, and what can you tell me
> about your experiences with your (purchased) nanoscope(s)?
>
> Sincerely,
>
> George
> p.s. Please no need to clutter up the listserv with other people's
> nanoscope references - I know how to use PubMed. For that matter, I've
> replicated the results of the following two papers on my confocal's:
>
>   Subdiffraction fluorescence imaging of biomolecular structure and
>   distributions with quantum dots. </pubmed/20600360>
>
>   Heidbreder M, Endesfelder U, van de Linde S, Hennig S, Widera D,
>   Kaltschmidt B, Kaltschmidt C, Heilemann M.
>
>   Biochim Biophys Acta. 2010 Oct;1803(10):1224-9. Epub 2010 Jun
>   23.PMID: 20600360
>
>
>   Quantum dot triexciton imaging with three-dimensional subdiffraction
>   resolution. </pubmed/19453186>
>
>   Hennig S, van de Linde S, Heilemann M, Sauer M.
>
>   Nano Lett. 2009 Jun;9(6):2466-70.PMID: 19453186
>
> I have used <1 Airy unit pinhole to improve triexciton resolution further.
> I have not tried doing 3D deconvolution on the data.
>
>
> --
>
>
> George McNamara, PhD
> Analytical Imaging Core Facility
> University of Miami
>