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At the Italian Institute of Technology, we got a Leica STED-CW and home built an FPALM on an inverted Nikon thanks to software sharing from Sam Hess Lab, University of Maine. We home built a WLL and a CW STED controlled by MPI Nanobiophotonics software. We are currently interested n the Nikon N-STORM.
All the best
Alby
Il giorno 29/gen/2011, alle ore 17.19, Christophe Leterrier ha scritto:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> The Bordeaux Imaging Center in Bordeaux, France has a STED and a TIRF/PALM
> http://www.bic.u-bordeaux2.fr/
>
> PALM/STORM setups are becoming more common (I'm aware of 2 setups already
> running in Marseille), because (at least this is what the people who did it
> told me) it is quite easy to add to an existing TIRF setup (provided you
> find software for the detection and localisation of individual fluorophores,
> but there is now an available ImageJ plugin for that).
>
>
> --
> Christophe Leterrier
> Postdoc
> INSERM UMR641 // Ionic channels Lab
> IFR Jean Roche, Mediterranée University
> Marseille, France
> [log in to unmask]
>
>
>
>
> On Sat, Jan 29, 2011 at 16:52, G. Esteban Fernandez <
> [log in to unmask]> wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>>
>> UCLA has a STED
>>
>> http://clms.cnsi.ucla.edu/cnsi/clms/equipment-list?search_lab=6791
>> <http://clms.cnsi.ucla.edu/cnsi/clms/equipment-list?search_lab=6791>
>> http://www.cnsi.ucla.edu/staticpages/core-facilities#alms
>>
>>
>> <http://www.cnsi.ucla.edu/staticpages/core-facilities#alms>
>>
>> On Sat, Jan 29, 2011 at 7:32 AM, George McNamara
>> <[log in to unmask]>wrote:
>>
>>> *****
>>> To join, leave or search the confocal microscopy listserv, go to:
>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>>> *****
>>>
>>> Dear Confocal listserv,
>>>
>>> Who has purchased a fluorescence nanoscope? What can you tell me about
>> your
>>> experiences - either here or privately?
>>>
>>> I see 11 OMX labs listed at http://www.api.com/omx-labs.asp (may be out
>> of
>>> date or some customers may be shy).
>>>
>>> I am aware of STED systems in the USA at Yale Univ, NIH and I was told
>> San
>>> Diego (have not found where), and possibly UC Denver. Paul French
>> apparently
>>> did his own upgrade of a Leica SP2 (
>>> http://www.focusonmicroscopy.org/2008/PDF/070_Auksorius.pdf and a Leica
>>> Scientific Forum video ... see also related work at
>>> ftp://ftp.espci.fr/incoming/Gilles/INSERM-STED-x6.pdf ).
>>>
>>> I am also aware of one 4pi microscope in the USA.
>>>
>>> So, who has N-SIM, N-STORM, SR-SIM, PALm, SR-200, and what can you tell
>> me
>>> about your experiences with your (purchased) nanoscope(s)?
>>>
>>> Sincerely,
>>>
>>> George
>>> p.s. Please no need to clutter up the listserv with other people's
>>> nanoscope references - I know how to use PubMed. For that matter, I've
>>> replicated the results of the following two papers on my confocal's:
>>>
>>> Subdiffraction fluorescence imaging of biomolecular structure and
>>> distributions with quantum dots. </pubmed/20600360>
>>>
>>> Heidbreder M, Endesfelder U, van de Linde S, Hennig S, Widera D,
>>> Kaltschmidt B, Kaltschmidt C, Heilemann M.
>>>
>>> Biochim Biophys Acta. 2010 Oct;1803(10):1224-9. Epub 2010 Jun
>>> 23.PMID: 20600360
>>>
>>>
>>> Quantum dot triexciton imaging with three-dimensional subdiffraction
>>> resolution. </pubmed/19453186>
>>>
>>> Hennig S, van de Linde S, Heilemann M, Sauer M.
>>>
>>> Nano Lett. 2009 Jun;9(6):2466-70.PMID: 19453186
>>>
>>> I have used <1 Airy unit pinhole to improve triexciton resolution
>> further.
>>> I have not tried doing 3D deconvolution on the data.
>>>
>>>
>>> --
>>>
>>>
>>> George McNamara, PhD
>>> Analytical Imaging Core Facility
>>> University of Miami
>>>
>>
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