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For those of you who might be interested in just 
getting a quick overview of STORM/PALM as well as 
several other superresolution techniques, I've 
just posted the BioPhotonics article at our 
website, www.MicroscopyEducation.com.  Just click 
on the "Library" and look for the listing.

Best regards,
Barbara Foster, President and Sr. Consultant

Microscopy/Microscopy Education
7101 Royal Glen Trail, Suite A
McKinney TX 75070
P: (972)924-5310  Skype: fostermme
W: www.MicroscopyEducation.com



At 05:59 PM 1/31/2011, you wrote:
>I've built STORM/PALM system and can confirm that it's not t
>*****
>To join, leave or search the confocal microscopy listserv, go to:
>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>*****
>
>Hi David, I've built STORM/PALM system and can 
>confirm that it's not too difficult. That said, 
>every system will usually be quite different, 
>depending on the specific requirements and 
>abilities making a 'one size fits all' 
>instruction manual very difficult to write. In 
>general it'd be fair to say that some 
>optics/laser design & alignment experience is 
>desirable when it comes to getting the hardware 
>side working, and it might be worth getting 
>someone from the local physics department to 
>help you. Programming experience is also a plus, 
>although something like 'QuickPALM' (as 
>mentioned by John) might give you a leg up 
>(you'd probably still need to do a bit of 
>tweaking to get everything working on your 
>system). I'm personally not a huge fan of 
>ImageJ/umanager/ Java in general, so would 
>probably look for another solution, but this 
>might be personal preference. We use custom, in 
>house, software which I think is a lot more 
>mature, but which is currently not particularly 
>well documented. It also pays to give some 
>thought to what variants of PALM/STORM you want 
>to support as this has a huge influence on the 
>setup. If your users are looking at fixed 
>specimens with antibody labelling, something 
>like dSTORM is probably the easiest to implement 
>(single laser, no complicated switching 
>patterns, can get away without software control 
>of shutters etc ...). The two aspects of the 
>hardware that you can't really get off the shelf 
>components for and are probably going to need to 
>play with are: -  the laser coupling (commercial 
>TIRF couplers typically give too little power 
>over too large a field of view), - the focus 
>mechanism (most commercial z-drives show quite a 
>lot of drift - so you're going to either need 
>some form of active stabilisation, or to 
>redesign the focus mechanism - we ended up using 
>a PIFoc piezo focusser on a custom metal bracket 
>& doing away with the microscopes focus 
>mechanism completely). If you've got any 
>specific questions, you're welcome to get in 
>touch, cheers, David ----- Original Message ---- 
>From: David Burk <[log in to unmask]> To: 
>[log in to unmask] Sent: Tue, 1 
>February, 2011 4:05:53 AM Subject: Building a 
>STORM/FPALM ***** To join, leave or search the 
>confocal microscopy listserv, go to: 
>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy 
>***** Since the topic of home built 
>super-resolution systems has been brought up I 
>was wondering if anyone had a very simple "Super 
>Res for Dummies" type manual for building such a 
>system.  While I feel quite capable of following 
>directions and assembling components together I 
>have a certain degree of trepidation when it 
>comes to figuring out how to drive all the 
>components software-wise.  I will admit I lack 
>programming experience and limit myself to 
>making ImageJ macros (poorly) or Cell Profiler 
>pipelines - not writing code in Matlab to open 
>shutters and such.  I am aware that many 
>articles describe their setups and give overview 
>diagrams but, from a true schematic standpoint, 
>they lack sufficient detail for someone as 
>rigidly OCD as myself.  Perhaps there is a class 
>or course offered somewhere that covers this 
>"roll your own" approach and I have yet to 
>convince Google to divulge that 
>information.  Along the same lines, I am very 
>interested in trying to construct an optical 
>projection tomography system for our facility 
>and, again, while I know many labs have built 
>their own systems and published details of them, 
>some critical details elude me.  Have any 
>listers built their own OPT rig and, if so, 
>could you provide a detailed component list as 
>well as assembly instructions?  Personally, I 
>would be more than willing to come visit a lab 
>that has implemented their own solution to the 
>FPALM/STORM and/or OPT method if they wouldn't 
>mind spending a little time answering what they 
>most likely would think are silly questions (and 
>would allow me to take pictures of their 
>system). David David H. Burk, PhD Cell Biology 
>and Bioimaging Core Pennington Biomedical 
>Research Center Baton Rouge, LA 70808 
>-----Original Message----- From: Confocal 
>Microscopy List 
>[mailto:[log in to unmask]] On 
>Behalf Of Alberto Diaspro Sent: Saturday, 
>January 29, 2011 10:47 AM To: 
>[log in to unmask] Subject: Re: 
>Who has purchased a fluorescence nanoscope? 
>***** To join, leave or search the confocal 
>microscopy listserv, go to: 
>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy 
>***** At the Italian Institute of Technology, we 
>got a Leica STED-CW and home built an FPALM on 
>an inverted Nikon thanks to software sharing 
>from Sam Hess Lab, University of Maine. We home 
>built a WLL and a CW STED controlled by MPI 
>Nanobiophotonics software. We are currently 
>interested n the Nikon N-STORM. All the best 
>Alby Il giorno 29/gen/2011, alle ore 17.19, 
>Christophe Leterrier ha scritto: > ***** > To 
>join, leave or search the confocal microscopy 
>listserv, go to: > 
>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy  
> > ***** > > The Bordeaux Imaging Center in 
>Bordeaux, France has a STED and a > TIRF/PALM 
>http://www.bic.u-bordeaux2.fr/ > > PALM/STORM 
>setups are becoming more common (I'm aware of 2 
>setups > already running in Marseille), because 
>(at least this is what the > people who did it 
>told me) it is quite easy to add to an existing 
>TIRF > setup (provided you find software for the 
>detection and localisation > of individual 
>fluorophores, but there is now  an available 
>ImageJ plugin for >that). > > > -- > Christophe 
>Leterrier > Postdoc > INSERM UMR641 // Ionic 
>channels Lab > IFR Jean Roche, Mediterranée 
>University Marseille, France > 
>[log in to unmask] > > > > > On 
>Sat, Jan 29, 2011 at 16:52, G. Esteban Fernandez 
>< > [log in to unmask]> wrote: > >> 
>***** >> To join, leave or search the confocal 
>microscopy listserv, go to: >> 
>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy  
> >> ***** >> >> UCLA has a STED >> >> 
>http://clms.cnsi.ucla.edu/cnsi/clms/equipment-list?search_lab=6791  
> >> 
><http://clms.cnsi.ucla.edu/cnsi/clms/equipment-list?search_lab=6791>  
> >> 
>http://www.cnsi.ucla.edu/staticpages/core-facilities#alms  
> >> >> >> 
><http://www.cnsi.ucla.edu/staticpages/core-facilities#alms>  
> >> >> On Sat, Jan 29, 2011 at 7:32 AM, George 
>McNamara >> 
><[log in to unmask]>wrote: >> >>> 
>***** >>> To join, leave or search the confocal 
>microscopy listserv, go to: >>> 
>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy  
> >>> ***** >>> >>> Dear Confocal 
>listserv, >>> >>> Who has purchased a 
>fluorescence nanoscope? What can you tell me >>> 
>about >> your >>> experiences - either here or 
>privately? >>> >>> I see 11 OMX labs listed at 
>http://www.api.com/omx-labs.asp (may be >>> 
>out >> of >>> date or some customers may be 
>shy). >>> >>> I am aware of STED systems in the 
>USA at Yale Univ, NIH and I was >>> told >> 
>San >>> Diego (have not found where), and 
>possibly UC Denver. Paul French >> 
>apparently >>> did his own upgrade of a Leica 
>SP2 ( >>> 
>http://www.focusonmicroscopy.org/2008/PDF/070_Auksorius.pdf 
>and a >>> Leica Scientific Forum video ... see 
>also related work at >>> 
>ftp://ftp.espci.fr/incoming/Gilles/INSERM-STED-x6.pdf 
>). >>> >>> I am also aware of one 4pi microscope 
>in the USA. >>> >>> So, who has N-SIM, N-STORM, 
>SR-SIM, PALm, SR-200, and what can you >>> 
>tell >> me >>> about your experiences with your 
>(purchased) nanoscope(s)? >>> >>> 
>Sincerely, >>> >>> George >>> p.s. Please no 
>need to clutter up the listserv with other 
>people's >>> nanoscope references - I know how 
>to use PubMed. For that matter, >>> I've 
>replicated the results of the following two 
>papers on my confocal's: >>> >>> Subdiffraction 
>fluorescence imaging of biomolecular structure 
>and >>> distributions with quantum dots. 
></pubmed/20600360> >>> >>> Heidbreder M, 
>Endesfelder U, van de Linde S, Hennig S, Widera 
>D, >>> Kaltschmidt B, Kaltschmidt C, Heilemann 
>M. >>> >>> Biochim Biophys Acta. 2010 
>Oct;1803(10):1224-9. Epub 2010 Jun >>> 23.PMID: 
>20600360 >>> >>> >>> Quantum dot triexciton 
>imaging with three-dimensional 
>subdiffraction >>> resolution. 
></pubmed/19453186> >>> >>> Hennig S, van de 
>Linde S, Heilemann M, Sauer M. >>> >>> Nano 
>Lett. 2009 Jun;9(6):2466-70.PMID: 
>19453186 >>> >>> I have used <1 Airy unit 
>pinhole to improve triexciton resolution >> 
>further. >>> I have not tried doing 3D 
>deconvolution on the data. >>> >>> >>> 
>-- >>> >>> >>> George McNamara, PhD >>> 
>Analytical Imaging Core Facility >>> University 
>of Miami >>> >> ISTITUTO ITALIANO DI TECNOLOGIA 
>Prof. Alberto Diaspro Scientific Head 
>Nanophysics Via Morego, 30 16163 Genova Tel: 
>+39-010.71.781.503 Fax +39-010-72.03.21 Mobile 
>+39-3666719968 www.iit.it [log in to unmask]