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Hi,
a year ago Olympus has launched a 60x lens which is corrected for 405 nm in contrast to the 435 nm commenly corrected for.
http://www.microscopy-analysis.com/news/olympus-launches-super-corrected-60x-14-na-objective-lensc
They claim 0.1 - 0.2 micrometer chromatic shift from 405 - 650 nm.
best wishes
Andreas
-----Original Message-----
From: George McNamara <[log in to unmask]>
To: [log in to unmask]
Sent: Tue, 11 Jan 2011 2:17
Subject: Re: Glycerol Objectives - experience with
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Hi Jeremy,
A lot cheaper to optimize the specimen refractive index tl an oil immersion lens.
$63.20 ... 2,2'-thiodiethanol, 1 liter (Staudt ... Hell, Microsc Res Tech).
Pricing from one of the microscope companies (other companies will be similar):
$12,445 ... 63x/1.2 water w/correction collar (coverglass thickness 0.14 to 0.19 mm), apochromat
$10,732 ... 63x/1.3 Imm Corr DIC (corr = coverglass thickness 0.15 to 0.19 mm)
$6,723 or $7,065 ... 63x/1.4 oil iris NA 0.7 to 1.4), plan apochromat
$6,075 ... 63x/1.4 oil DIC (for coverglass 0.17 mm)
So, you can buy the last objective lens (DIC prisms not included) and a whole lot of TDE for a lot less than the other lenses. See Stan Vitha's email message to the listserv for his recommendation on gradual transition into TDE. Ignoring bulk volume discount, you could buy 1,000 bottles of 1 liter TDE plus the last objective lens for about the same price as the first lens listed above. It will be a long time to use up 1,000 liters of TDE!
Philippe Clemenceau <[log in to unmask]> sent me a PDF of his company's adaptive optics device for microscopy (he sent an email to the listserv recently). Ask him for it. See also http://www.imagine-optic.com/iop_applications_adaptive-optics_microscopy-life-sciences_en.php
I am looking forward to finding out the pricing (and compatibility with cameras and imaging software) for use with improving standard widefield fluorescence images. Could be a lot more useful than the manual (and therefore pretty useless for thick specimens) correction collars on various lenses. Philippe mentioned that his company's device can enable some types of 3D PALM/STORM, and also reduce spherical aberrations for both widefield fluorescence microscopy and biplane FPALM nanoscopy. 3i has also been offering the "SAC - spherical aberration corrector" for a couple of years.
enjoy,
George
TDE price from http://www.sigmaaldrich.com/catalog/Lookup.do?N5=All&N3=mode+matchpartialmax&N4=thiodiethanol&D7=0&D10=thiodiethanol&N1=S_ID&ST=RS&N25=0&F=PR
On 1/10/2011 3:39 AM, Jeremy Adler wrote:
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> To join, leave or search the confocal microscopy listserv, go to:
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>
> We are considering buying a new confocal for examining fixed and > potentially thick biological specimens.
> Given the need to have to an RI match between the specimen, mounting > medium and immersion medium, I want to ask about the pros and cons of > glycerol objectives.
>
> Oil objectives appear to have no obvious advantages, the higher RI > will only apply if specimens are mounted in a medium with a RI close > to that of oil - while most media seem to have lower RIs. Why would > anyone choose an oil objective and which mounting media work for > thick specimens ?
>
> A possible disadvantage of glycerol objectives is that glycerol is > hygroscopic which could change its RI. Are there any RI equivalent > immersion media ?.
>
> So the pros and cons of oil or glycerol objectives.
>
>
>
>
>
>
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> Jeremy Adler
> Genetics & Pathology
> Rudbeckslaboratoriet
> Daghammersköljdsväg 20
> 751 85 Uppsala
> Sweden
>
> 0046 (0)18 471 4607
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