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I've had good success with a cheap machine vision camera and a standard tube
lens.  If you have a camera port the tube lens will probably already be
installed there.  The trick will be getting a wide enough field of view.
 Generally a larger CCD array on the camera will help with this.

Craig


On Fri, Jan 14, 2011 at 10:12 AM, Steffen Dietzel <[log in to unmask]>wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Simon,
>
> if you put a little screen in front of the eye piece, some centimeters
> away, at the right distance you'll get a sharp image. You can demonstrate
> the effect easily with a bright field microscope and a piece of paper. You
> probably could borrow a screen from somebody teaching basic optics at the
> physics department.
>
> With similar strategy, you could mount a digital consumer camera in front
> of the eye piece. A respective adapter is shown in the image on this page
> (bottom image):
>
> http://www.doerrfoto.de/Produkte/Article.aspx?productline=192&article=566556
> (make sure its all in one line in your browser's address field)
> The adapter is clamped around the eye piece and the camera then centered. I
> got such an adapter from a local photography/optics shop. Works nice for
> bright field, e.g. for preparation microscopes. No experience with
> fluorescence though.
>
> Of course, for fluorescence sensitivity will be an issue in both cases and
> I wouldn't be too optimistic. But since the attached costs are rather
> modest, maybe it's worth trying.
>
> Steffen
>
>
>
>
> On 14.01.2011 15:32, Watkins, Simon C wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>>
>> Folks, I am about to install an advanced imaging system (sweptfield
>> confocal and widefield imaging combo) in a biosafety level 3 facility.  To
>> work in the facility one has to suit up and wear a rigid face mask, as the
>> cells/pathogens will be on the scope and may be open to the environment.
>>  This means that the eyepieces of the scope are essentially useless.  I
>> wonder if any other listers have dealt with this problem and what their
>> solution was? Obviously the widefield camera will help a lot, but it doesnt
>> allow survey of the full field of view, as we are doing mostly flourescence
>> a video camera isnt much use.... back in the day, there were some screen
>> solutions....
>> Looking for creative ideas
>> S.
>>
>> Simon C. Watkins Ph.D, FRC Path
>> Professor and Vice Chair Cell Biology and Physiology
>> Professor Immunology Director Center for Biologic Imaging
>> BSTS 225
>> University of Pittsburgh
>> 3500 Terrace St
>> Pittsburgh PA 15261
>> 412-352-2277
>> www.cbi.pitt.edu<http://www.cbi.pitt.edu>
>>
>>
>
> --
> ------------------------------------------------------------
> Steffen Dietzel, PD Dr. rer. nat
> Ludwig-Maximilians-Universität München
> Walter-Brendel-Zentrum für experimentelle Medizin (WBex)
> Head of light microscopy
>
> Mail room:
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>