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If I understand well, the dilemma is that the camera in the side port would not let you see the complete field of view to efficiently search the sample as you can do with eye pieces... using the Confocal monitor to search the sample has the same problem of limited field of view and adds the problem of slow refresh rate and unnecessary photobleaching... plus is annoying...
-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of S. Brunet
Sent: Friday, January 14, 2011 12:50 PM
To: [log in to unmask]
Subject: Re: BSL3 microscopy
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Hello:
We are not BSL3, but we set up a camera + adapter at the eyepiece from Martin
Microscope (no financial affiliation) on our LSM410. I can see many
fluorescent samples this way.
You could try a higher end camera and send the output to a monitor (some cameras
can be controlled using the computer software).
My biggest problem with the camera I have is the automatic focussing which can
correct for mis-focussing the objective (I can control that to an extent but I
prefer manual camera sometimes).
Vignetting will happen for a large field of view. I can't remember off hand how
the field view compares to the eyepiece (probably a bit less).
I am not sure if this is the option you referred to in your post (that you say
would not work fo you).
I would assume that most new microscopes with a digital imaging camera would
suit your purpose... so I am trying to understand the problem...
Good luck,
Sophie
____________________________________________________
Sophie M. K. Brunet, Ph. D.
Research Officer
Optical Spectroscopy, Laser Systems and Applications
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306-966-1719 (office) 306-966-1702 (fax)
____________________________________________________
Saskatchewan Structural Sciences Centre
University of Saskatchewan
Thorvaldson Bldg.
110 Science Place
Saskatoon, Sk S7N 5C9
____________________________________________________
Quoting "Watkins, Simon C" <[log in to unmask]>:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Folks, I am about to install an advanced imaging system (sweptfield confocal
> and widefield imaging combo) in a biosafety level 3 facility. To work in the
> facility one has to suit up and wear a rigid face mask, as the
> cells/pathogens will be on the scope and may be open to the environment.
> This means that the eyepieces of the scope are essentially useless. I wonder
> if any other listers have dealt with this problem and what their solution
> was? Obviously the widefield camera will help a lot, but it doesnt allow
> survey of the full field of view, as we are doing mostly flourescence a video
> camera isnt much use.... back in the day, there were some screen
> solutions....
> Looking for creative ideas
> S.
>
> Simon C. Watkins Ph.D, FRC Path
> Professor and Vice Chair Cell Biology and Physiology
> Professor Immunology Director Center for Biologic Imaging
> BSTS 225
> University of Pittsburgh
> 3500 Terrace St
> Pittsburgh PA 15261
> 412-352-2277
> www.cbi.pitt.edu<http://www.cbi.pitt.edu>
>
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