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January 2011

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Confocal Microscopy List <[log in to unmask]>
Date:
Sun, 16 Jan 2011 09:46:30 -0500
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Confocal Microscopy List <[log in to unmask]>
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Re: BSL3 microscopy
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*****
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Hi Simon,

Leave the eyepieces in the drawer and teach your users to use the live 
camera images. Would also be useful to have an identical microscope in 
the main lab that users can learn on and adapt to.

You can also take a tip from Sedat lab's and Applied Precision's use of 
a "scout scope". More practically, in your BSL3 system, low 
magnification lens and/or motorized XY(Z) tile scanning.

You could take this even further by having a duplicate computer monitor, 
keyboard, mouse, etc, outside the BSL3 space. Preferably with the 
microscope on the other side of a window from the non-3 station. Same 
ideas would apply to a microscope in a GMP facility (which is the idea 
came from).

George

p.s. Are you really studying flour in there? ... flourescence

On 1/14/2011 9:32 AM, Watkins, Simon C wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Folks, I am about to install an advanced imaging system (sweptfield confocal and widefield imaging combo) in a biosafety level 3 facility.  To work in the facility one has to suit up and wear a rigid face mask, as the cells/pathogens will be on the scope and may be open to the environment.  This means that the eyepieces of the scope are essentially useless.  I wonder if any other listers have dealt with this problem and what their solution was? Obviously the widefield camera will help a lot, but it doesnt allow survey of the full field of view, as we are doing mostly flourescence a video camera isnt much use.... back in the day, there were some screen solutions....
> Looking for creative ideas
> S.
>
> Simon C. Watkins Ph.D, FRC Path
> Professor and Vice Chair Cell Biology and Physiology
> Professor Immunology Director Center for Biologic Imaging
> BSTS 225
> University of Pittsburgh
> 3500 Terrace St
> Pittsburgh PA 15261
> 412-352-2277
> www.cbi.pitt.edu<http://www.cbi.pitt.edu>
>
>

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