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A few years ago we evaluated the Leica 63X glycerol objective side-by-side with the water immersion one on the AOBS SP2 for work with live tissue and found the glycerol one to be enough of an improvement to purchase it.
-Michael C.
-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Rosemary White
Sent: Monday, January 10, 2011 4:48 PM
To: [log in to unmask]
Subject: Re: Glycerol Objectives - experience with
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Dear Lloyd,
Does the 63x glycerol objective provide substantial improvements over the
63x water (with correction collar) for live tissues? We have one of the
"blue" Leica 63x objectives, corrected for the blue-UV end of the spectrum
(though only have 405 laser), which is great except when you want to image
both red and blue emission....
thanks,
Rosemary White
Dr Rosemary White
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia
T 61 2 6246 5475
F 61 2 6246 5334
E [log in to unmask]
On 11/01/11 6:21 AM, "Lloyd Donaldson" <[log in to unmask]>
wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Jeremy
>
> We have a Leica 63x glycerol objective with adjusting ring which gives very
> noticeable improvement over 63x oil which we also have. We also have a 20x
> multi immersion objective that can use glycerol. We have both oil and glycerol
> objectives, although we prefer glycerol as a mounting medium it is
> incompatible with some of our samples because of induced swelling so we have
> to mount in oil for those samples. We also have the oil lens because it is UV
> compatible - we have a 355 nm laser.
>
>
> Dr Lloyd Donaldson
>
> Senior Scientist, Project Leader - Microscopy/Wood Identification
> Scion - Next Generation Biomaterials
> Private Bag 3020, Rotorua
> New Zealand 3010
>
> Ph: 64 7 343 5581
>
>
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[log in to unmask]] On
> Behalf Of Jeremy Adler
> Sent: Monday, 10 January 2011 9:39 p.m.
> To: [log in to unmask]
> Subject: Glycerol Objectives - experience with
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> We are considering buying a new confocal for examining fixed and
> potentially thick biological specimens.
> Given the need to have to an RI match between the specimen, mounting
> medium and immersion medium, I want to ask about the pros and cons of
> glycerol objectives.
>
> Oil objectives appear to have no obvious advantages, the higher RI
> will only apply if specimens are mounted in a medium with a RI close
> to that of oil - while most media seem to have lower RIs. Why would
> anyone choose an oil objective and which mounting media work for
> thick specimens ?
>
> A possible disadvantage of glycerol objectives is that glycerol is
> hygroscopic which could change its RI. Are there any RI equivalent
> immersion media ?.
>
> So the pros and cons of oil or glycerol objectives.
>
>
>
>
>
>
>
> Jeremy Adler
> Genetics & Pathology
> Rudbeckslaboratoriet
> Daghammersköljdsväg 20
> 751 85 Uppsala
> Sweden
>
> 0046 (0)18 471 4607
>
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