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Simon,

if you put a little screen in front of the eye piece, some centimeters 
away, at the right distance you'll get a sharp image. You can 
demonstrate the effect easily with a bright field microscope and a piece 
of paper. You probably could borrow a screen from somebody teaching 
basic optics at the physics department.

With similar strategy, you could mount a digital consumer camera in 
front of the eye piece. A respective adapter is shown in the image on 
this page (bottom image):
http://www.doerrfoto.de/Produkte/Article.aspx?productline=192&article=566556
(make sure its all in one line in your browser's address field)
The adapter is clamped around the eye piece and the camera then 
centered. I got such an adapter from a local photography/optics shop. 
Works nice for bright field, e.g. for preparation microscopes. No 
experience with fluorescence though.

Of course, for fluorescence sensitivity will be an issue in both cases 
and I wouldn't be too optimistic. But since the attached costs are 
rather modest, maybe it's worth trying.

Steffen



On 14.01.2011 15:32, Watkins, Simon C wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Folks, I am about to install an advanced imaging system (sweptfield confocal and widefield imaging combo) in a biosafety level 3 facility.  To work in the facility one has to suit up and wear a rigid face mask, as the cells/pathogens will be on the scope and may be open to the environment.  This means that the eyepieces of the scope are essentially useless.  I wonder if any other listers have dealt with this problem and what their solution was? Obviously the widefield camera will help a lot, but it doesnt allow survey of the full field of view, as we are doing mostly flourescence a video camera isnt much use.... back in the day, there were some screen solutions....
> Looking for creative ideas
> S.
>
> Simon C. Watkins Ph.D, FRC Path
> Professor and Vice Chair Cell Biology and Physiology
> Professor Immunology Director Center for Biologic Imaging
> BSTS 225
> University of Pittsburgh
> 3500 Terrace St
> Pittsburgh PA 15261
> 412-352-2277
> www.cbi.pitt.edu<http://www.cbi.pitt.edu>
>


-- 
------------------------------------------------------------
Steffen Dietzel, PD Dr. rer. nat
Ludwig-Maximilians-Universität München
Walter-Brendel-Zentrum für experimentelle Medizin (WBex)
Head of light microscopy

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