*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

I use the Chroma slides and fluorescein solution at known concentrations.
 Use a water dipping lens with the solution and image away from either the
bottom of the dish or the surface of the water.  You want to be about middle
depth.  It won't bleach and as long as you weight it out carefully before
putting it into solution you can repeatably create it.

Craig


On Fri, Jan 14, 2011 at 5:39 PM, Mark Cannell <[log in to unmask]>wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> How about Fluorescent plastic slides -I got some free from Chroma.
>
> Cheers
>
>
>
> On 15/01/2011, at 12:34 PM, Boswell, Carl A - (cboswell) wrote:
>
>  Hi all,
>> I'm looking for some trustworthy, permanent standard(s) to use in a MP
>> system to monitor the condition of detectors and the system in general.
>> The goal is a fluorescent source that is immutable.  This could done either
>> by having unrestricted replacement of bleached label with unbleached label
>> (i.e. a solution), or a uniform and very large solid, so that it is unlikely
>> that any one tiny volume will be imaged repeatedly.   We've tried several
>> iterations of an aqueous solution of label, but sealing the prep does not
>> prevent eventual evaporation.  Plus, there seems to be some modification of
>> FITC, for example,  in solution over time (a month) regardless of exposure
>> to light.  I'm not sure I can trust the consistency of colored plastic
>> slides, since they are not manufactured with this role in mind.  One
>> possibility is uranyl glass slides, but Corning says they don't make these
>> any more.  Does anyone know a source?  We're also considering quantum dots
>> in something like immersion oil , in a sealed chamber but I don't have any
>> experience with these labels in a hydrophobic environment.
>>
>> Thanks for your insights.
>> C
>>
>>
>> Carl A. Boswell, Ph.D.
>> Molecular and Cellular Biology
>> Univ. of Arizona
>> 520-954-7053
>> FAX 520-621-3709
>>
>>
>>