LISTSERV - CONFOCALMICROSCOPY Archives

CONFOCALMICROSCOPY Archives

April 2011

CONFOCALMICROSCOPY@LISTS.UMN.EDU

	LISTSERV Archives
	CONFOCALMICROSCOPY Home
	CONFOCALMICROSCOPY April 2011

	Log In
	Register

	Subscribe or Unsubscribe

	Search Archives

Options:	Use Monospaced Font Show Text Part by Default Condense Mail Headers
Message:	[<< First] [< Prev] [Next >] [Last >>]
Topic:	[<< First] [< Prev] [Next >] [Last >>]
Author:	[<< First] [< Prev] [Next >] [Last >>]

Mime-Version:	1.0 (Apple Message framework v1084)
Sender:	Confocal Microscopy List <[log in to unmask]>
Subject:	Fluorophores for ground state depletion
From:	Colin Rickman <[log in to unmask]>
Date:	Tue, 26 Apr 2011 21:02:48 +0100
Content-Type:	text/plain; charset=us-ascii
Content-Transfer-Encoding:	quoted-printable
Reply-To:	Confocal Microscopy List <[log in to unmask]>
Parts/Attachments:	text/plain (28 lines)

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Hi

Can anybody recommend a fluorophore for use in ground state depletion microscopy. I have been using Alexa 488 with some success and am able to acquire data at the single molecule level for localisation. However, I would like to know if there are better options within the Alexa range or in other organic dyes with enhanced propensity to enter triplet states, longer duration in the triplet state, etc. that make them better candidates.

Hope someone can help.

Regards

Colin

Dr Colin Rickman
Life Science Interface
Department of Chemistry (WP 2.03)
School of Engineering and Physical Sciences
Heriot-Watt University
Edinburgh
EH14 4AS

Tel: +44 131 4514193 (Office)

http://www.lifescienceinterface.hw.ac.uk
http://www.eps.hw.ac.uk/departments/chemistry/cr.htm

ATOM RSS1 RSS2

LISTS.UMN.EDU