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Lipid droplets in stellate (Ito) cells are highly autofluorescent due to their content of esters of retinoic acid. These are probably your structures. 

--
John J. Lemasters, MD, PhD
Professor and GlaxoSmithKline Distinguished Endowed Chair
Director, Center for Cell Death, Injury and Regeneration
Departments of Pharmaceutical & Biomedical Sciences and Biochemistry & Molecular Biology
Medical University of South Carolina
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-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Jean-Pierre CLAMME
Sent: Tuesday, April 05, 2011 9:06 PM
To: [log in to unmask]
Subject: Experience with liver tissue autofluorescence ?

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Hi,

I'm looking for someone having experience with confocal imaging of liver tissue. I'm imaging mouse liver tissue and I have some issues with autofluorescence in the cy3 channel coming from structure appearing like vesicle in hepatocytes. Those vesicles mainly show up in the Cy3 channel but are also visible in the FITC channel (depending on the power I use).
A spectral image with excitation at 488, shows a broad signal with a maximum around 580 nm.
Could someone comment on the origin of this fluorescence ? Is it possible that it is lipofuscine ? 

Thank you,

JP