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Based on their spectra, I wouldn't expect the fluorescence of esters of 
retinoids to be fluorescent in the Cy3 channel  but more in the DAPI 
channel. Also I mainly see those structures around the round nucleus of 
hepatocytes.


> Lipid droplets in stellate (Ito) cells are highly autofluorescent 
> due to their content of esters of retinoic acid. These are probably 
> your structures.

> --
> John J. Lemasters, MD, PhD
> Professor and GlaxoSmithKline Distinguished Endowed Chair
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> Departments of Pharmaceutical & Biomedical Sciences and Biochemistry
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> Medical University of South Carolina
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> 
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[log in to unmask]
> EDU] On Behalf Of Jean-Pierre CLAMME
> Sent: Tuesday, April 05, 2011 9:06 PM
> To: [log in to unmask]
> Subject: Experience with liver tissue autofluorescence ?

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> Hi,

> I'm looking for someone having experience with confocal imaging of 
> liver tissue. I'm imaging mouse liver tissue and I have some issues 
> with autofluorescence in the cy3 channel coming from structure 
> appearing like vesicle in hepatocytes. Those vesicles mainly show up
> in the Cy3 channel but are also visible in the FITC channel 
> (depending on the power I use).
> A spectral image with excitation at 488, shows a broad signal with a
> maximum around 580 nm.
> Could someone comment on the origin of this fluorescence ? Is it 
> possible that it is lipofuscine ?

> Thank you,

> JP