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  I would bet that both the 350 and 700 nm peaks are due to scattering.  
You can easily check if the 700nm peak is due to scattering by putting a 
UV-blocking longpass filter on the detection arm of the fluorimeter.

On 4/28/2011 8:23 AM, S. Brunet wrote:
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> Hi,
> Is your emission peak at 700nm from excitation at 350nm?
> If so, did you verify that it is not due to 2nd order diffraction of 350nm from
> the emission monochromator?
> Bye,
> Sophie
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>
> Quoting charu tanwar<[log in to unmask]>:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>>
>> Dear Michael
>>   
>> We got the excitation peak and emission peak data for these nanoparticles
>> from flourimeter (we scanned the particles in the range 200nm - 700nm and we
>> got one excitation peak at 350nm. Subsequently we got 2 emission peaks at
>> 350nm and 700nm). After confirming the excitation and emission spectra we
>> need to study theses particles insde cellualr system through confocal
>> microscopy.
>>
>> CHARU TANWAR
>> Imaging Specialist
>> Advanced Instrumentation Research Facility
>> Jawaharlal Nehru University
>> New Delhi 110067
>> India.
>>
>> --- On Wed, 27/4/11, Cammer, Michael<[log in to unmask]>  wrote:
>>
>>
>> From: Cammer, Michael<[log in to unmask]>
>> Subject: Re: same excitation and emission peaks
>> To: [log in to unmask]
>> Date: Wednesday, 27 April, 2011, 7:31 PM
>>
>>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
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>>
>> Are you seeing reflectance?  We used to use the confocal in reflectance mode
>> to look at lead ATPase reaction product in liver and nickle enhanced gold
>> immuno.
>>
>> _________________________________________
>> Michael Cammer, Assistant Research Scientist
>> Skirball Institute of Biomolecular Medicine
>> Lab: (212) 263-3208  Cell: (914) 309-3270
>>
>> ________________________________________
>> From: Confocal Microscopy List [[log in to unmask]] On Behalf
>> Of Charu Tanwar [[log in to unmask]]
>> Sent: Wednesday, April 27, 2011 9:59 AM
>> To: [log in to unmask]
>> Subject: same excitation and emission peaks
>>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
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>>
>> Dear List
>>
>> Not a direct confocal related query.
>> Anyone please let me know whether any metallic nanoparticle with a definite
>> size
>> can have the same emission peak as its excitation peak???
>>
>> Thanks in advance
>>
>> Charu Tanwar
>> Imaging Specialist
>> Advanced Instrumentation Research Facility
>> Jawaharlal Nehru University
>> New Delhi
>> India.
>>
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