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Two other publications of interest for you:

Dickinson, M.E., et al., Multiphoton excitation spectra in biological samples. Journal of Biomedical Optics, 2003. 8(3): p. 329-338.
Spiess, E., et al., Two-photon excitation and emission spectra of the green fluorescent protein variants ecfp, egfp and eyfp. Journal of Microscopy-Oxford, 2005. 217: p. 200-204. 

John Oreopoulos
Research Assistant
Spectral Applied Research
Richmond Hill, Ontario
Canada
www.spectral.ca


On 2011-04-05, at 7:46 PM, Boswell, Carl A - (cboswell) wrote:

> Probably the most comprehensive site would be at: http://www.spectra.arizona.edu/ .  We've compiled all the 2P data we can find, including the DRBIO data.  Simply select "2-photon cross-sections" under Dye category.  We hope to incorporate the ability to download any data the user chooses, soon.  This site has been assembled by Dr. Urs Utzinger at the U of A Biomedical Engineering Dept. and myself, with the code-writing help of Gary Carstensen in our Instruction/Access office, and includes much of the 1P data collected by Dr. George McNamara, U. of Miami, and myself.
> 
> Additional spectral data from optics, dyes, filters and light sources always welcome.
> 
> Cheers,
> C
> 
> 
> 
> Carl A. Boswell, Ph.D.
> Molecular and Cellular Biology
> Univ. of Arizona
> 520-954-7053
> FAX 520-621-3709
> 
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Farid Jalali
> Sent: Tuesday, April 05, 2011 8:30 AM
> To: [log in to unmask]
> Subject: Re: Multiphoton spectra of fluorescent dyes
> 
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
> 
> Hello Gary,
> 
> I have found the DRBIO website (Developmental Resource for Biophysical Imaging Opto-Electronics at Cornell- Warren Zipfel, Watt Webb and crew
> ) at http://www.drbio.cornell.edu/cross_sections.html to be an excellent resource for at least the 2P excitation cross sections. The following paper is good commonly used reagents. I have yet to find anything equivalent to the resources you mention however.
> 
> Two-photon fluorescence absorption and emission spectra of dyes relevant for cell imaging.
> J Microsc. 2002 Nov;208(Pt 2):108-15
> Bestvater F, Spiess E, Stobrawa G, Hacker M, Feurer T, Porwol T, Berchner-Pfannschmidt U, Wotzlaw C, Acker H.
> 
> 
> Cheers
> Farid
> 
> On Tue, Apr 5, 2011 at 8:03 AM, Gary G. Li <[log in to unmask]> wrote:
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>> 
>> Greetings,
>> 
>> For one photon excitation, the excitation and emission spectra of 
>> commonly used fluorescent dyes can be viewed by using the following tools:
>> 
>> * Invitrogen Fluorescence SpectraViewer, www.invitrogen.com
>> 
>> * BD Fluorescence Spectrum Viewer, www.bdbiosciences.com
>> 
>> I am wondering if we have similar tools for fluorophores with 
>> multiphoton excitation.
>> 
>> Many thanks
>> 
>> Gary G. Li, Ph.D.
>> 
>> Microscopy Core Manager
>> 
>> NIH/NIDA
>> 
>> 333 Cassell Drive, Suite 2100
>> 
>> Baltimore, MD 21224
>> 
>> [log in to unmask]
>> 
>> [log in to unmask] <[log in to unmask]>
>> 
>> 
>> <[log in to unmask]>
>> 
>