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So far we have been using one laser, which means we miss the beginning of the
timecourse after photoactivation because it takes several seconds for the Ti:S to
tune and mode-lock. So the experiments where 2P photoactivation has been
useful involve diffusion through gap junctions between cells in a cluster or chain
because the spread of the photoactivated dye between cells is relatively slow.
However, in June we will take delivery on a new 2P system with two Ti:S lasers
so we can do it simultaneously at two different wavelengths.
Kate