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I believe that SIM data is usually at least partly deconvolved already in the 
reconstruction process, although the extent of the deconvolution depends on the 
heritage of your SIM technique. I think all techniques involve at least a Weiner 
filtering step, and some might include a iterative optimisation as well. I'd 
thus be pretty cautious about performing any further deconvolution. If you do, 
you'd want to use very small beads (<50 nm) for your PSF measurements, which 
might make getting reliable (ie not noisy) PSF estimates difficult. Its also 
quite likely that the factors which end up limiting the resolution will relate 
to uneven bleaching ('burn in' of the mask) and mask errors (alignment errors 
and sample abberations which change the shape of the illumination pattern from 
what is expected). Deconvolution isn't going to help you here.

cheers,
David


----- Original Message ----
To: [log in to unmask]
Sent: Fri, 8 April, 2011 8:16:08 AM
Subject: Deconvolution of 3D SIM data

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Hi Everyone,

We are starting to process through  OMX data sets and we are writing for any 
thoughts on the use of deconvolution in these images. We have heard mixed 
things about whether or not it is valid...  Has anyone here deconvolved 3D SIM 
data?  Were actual or theoretical PSFs used? 

Much thanks,
A