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The problem with reconstruction of the SIM images is I think as with 
most other imaging techniques the signal to noise ratio of the raw 
images. An optimal deconvolution should there start on the raw images 
and not on the reconstructed images. As stated before all SIM algorithms 
do already contain some deconvolution, but I  think there is room for 
improvement. With a better knowledge of the deformations of the grid in 
the images and the effect in biological specimen the reconstruction 
based on deconvolution might improve. This needs a lot of testing with 
different biological samples or other tricks.

With kind regards,

Gert van Cappellen
Optical Imaging Centre
Erasmus MC (Erasmus Medical Centre)
Rotterdam
Netherlands


Op 7-4-2011 22:16, Amro Hamdoun schreef:
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> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi Everyone,
>
> We are starting to process through  OMX data sets and we are writing for any
> thoughts on the use of deconvolution in these images. We have heard mixed
> things about whether or not it is valid...  Has anyone here deconvolved 3D SIM
> data?  Were actual or theoretical PSFs used?
>
> Much thanks,
> A