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Gary,

Have you tried the DRBIO resource at Cornell that is run by Warren Zipfel now? They have a list of two-photon absorption cross-sections there that I find helpful. Its not exhaustive, but they have a number of the fluorescent proteins and alexa dyes listed there for example, and you can download the ascii data as well.

http://www.drbio.cornell.edu/cross_sections.html

Best wishes,
James.


On Apr 5, 2011, at 8:03 AM, Gary G. Li wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
> 
> Greetings,
> 
> For one photon excitation, the excitation and emission spectra of commonly
> used fluorescent dyes can be viewed by using the following tools:
> 
> * Invitrogen Fluorescence SpectraViewer, www.invitrogen.com
> 
> * BD Fluorescence Spectrum Viewer, www.bdbiosciences.com
> 
> I am wondering if we have similar tools for fluorophores with multiphoton
> excitation.
> 
> Many thanks
> 
> Gary G. Li, Ph.D.
> 
> Microscopy Core Manager
> 
> NIH/NIDA
> 
> 333 Cassell Drive, Suite 2100
> 
> Baltimore, MD 21224
> 
> [log in to unmask]
> 
> [log in to unmask] <[log in to unmask]>
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> 
> <[log in to unmask]>
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-----
James A.J. Fitzpatrick, Ph.D.
Core Director
	Waitt Advanced Biophotonics Center
The Salk Institute for Biological Studies
10010 North Torrey Pines Road
La Jolla, CA 92037

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