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Sun, 17 Apr 2011 12:45:21 -0700 |
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Nuno Moreno <[log in to unmask]> writes:
> Therefore, I would be very, very skeptical either on any deconvolution
> and quantification using SIM.
Actually if your data has high S/N then quantitation should be
possible. For 2D super-resolution with SIM you have an over specified
linear problem, 9 images to give 4x the data (double resolution in
x&y). With 3D-SIM, as produced by OMX scopes, the problem is similar, 15
images producing 8x as much information (double res in x,y & z). Go look
at the original Gustafson papers for the details.
The issue is getting high enough S/N, which in practical terms means low
enough bleaching while taking those 100+ images.
Ian
--
Ian Dobbie
Micron Imaging Facility Manager,
Biochemistry,
University of Oxford,
South Parks Road,
Oxford
OX1 3QU
Tel: 01865 613323
Email: [log in to unmask]
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