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Hi Mike and Pete

On Apr 15, 2011, at 7:02 AM, CONFOCALMICROSCOPY automatic digest system wrote:

> 
> Date:    Thu, 14 Apr 2011 10:27:38 +0200
> From:    Peter Gabriel Pitrone <[log in to unmask]>
> Subject: Re: FocalCheck test slide: Vendor Update
> 
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
> 
> Dear Mike,
> 
> This is great news! Thanks for listening to your costumers wishes.

Halleluja!

> 
> I still am of the opinion that each bead size should be on it's own
> slide. The "one slide fits all" method (with 6 positions in a straight
> line) needs to be discarded, because it is impossible to even reach the
> last two positions.

yes, and the beads need to be in the middle of the slide to be visible on an OMX
or other system / stage with restricted stage travel.

Also.. what exactly is the refractive index of the mounting medium, 
and what exactly is it anyway?

Can we get 50 nm diameter bead slides for super resolution structured illumination microscope systems like the omx?

100 or 170 nm are not small enough....

cheers

Dan

> 
> Thanks again!
> 
> and best regards,
> 
> Pete
> 
> 
> On Wed, 2011-04-13 at 16:01 -0500, Mike Ignatius wrote:
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>> 
>> Dear Listserve members,
>> 
>> We are pleased to announce that we were able to improve the mounted 
>> tetraspec beads products described in this colorful email string from over a 
>> year ago (with some comments extending back to 2005).    
>> 
>> Tertraspec beads are now mounted on the #1.5 coverslips, not the slides.  
>> 
>> It took some doing - overspray from the mask used to find the bead focal 
>> plane was our biggest unanticipated issue that delayed this so much.  Many 
>> apologies to anyone out there that remained on backorder.  But the product, 
>> T14792 and other bead mounted slides, like our intensity and size bead series, 
>> F36909, are now on the coverslip.  
>> 
>> Frankly they look terrific - wish I could post an image. 
>> 
>> Kind Regards,
>> 
>> Mike Ignatius
>> Molecular Probes/LifeTechnologies
>> 
>> On Fri, 26 Mar 2010 17:31:10 -0400, Ignatius, Mike 
>> <[log in to unmask]> wrote:
>> 
>>> Dear Listservors,
>>> 
>>> Thank you all very much for your contributions to this discussion.
>>> 
>>> We have always used #1.5 coverslips(which range from 160 to 190 
>> microns).  All biological objectives (except those with correction collars) are 
>> designed for these. Going to #1 coverslips would only narrow things by 30 
>> microns at best and defeat the optical correction factor. 
>>> 
>>> We continue to encourage do-it-yourselfers (DYI) slides as others have 
>> mentioned, and include protocols to do this.  We are very generous in the 
>> quantities of beads shipped - you can make dozens to hundreds of slides from 
>> one vial. However we developed the multiple bead slides as a cost savings to 
>> the customer needing more than one bead type.  Buying 10 bead preps is 2-5x 
>> what one of the prepared slides will cost.  
>>> 
>>> Unfortunately, the prepared slides are a quite a challenges to make in 
>> quantity.  We have strict QC standards - our scrap rate and thus costs are 
>> high.  We haven't implemented a QC step with oil objectives because of the 
>> damage to the slide that would entail.  As mentioned in an earlier post we are 
>> addressing this going forward (first up mounting on coverslips).  
>>> 
>>> We will refund anyone that is unable to see their beads on our slides of 
>> course.  Just call our Tech Support for a credit or replacement, at 800-955-
>> 6288, then selection 2, then selection 4 to request this.
>>> 
>>> Regards,
>>> 
>>> Mike Ignatius
>>> 
>>> Molecular Probes/Lifetechnologies
>>> 
>>> 
>>> 
>>> -----Original Message-----
>>> From: Confocal Microscopy List 
>> [mailto:[log in to unmask]] On Behalf Of Peter Pitrone
>>> Sent: Friday, March 26, 2010 6:03 AM
>>> To: [log in to unmask]
>>> Subject: Re: FocalCheck test slide
>>> 
>>> They sell these "test slides" for hundreds of dollars, yet they don't take the 
>> time to make them right?!?! They should be made with correct coverslips 
>> chosen for their thickness, deposited on them and then mounted on the glass 
>> slide... I see that there is a disconnect some where, how much does it cost 
>> them to produce these slides? 10 bucks maybe...
>>> 
>>> Pete
>>> 
>>> On Mar 26, 2010, at 12:25 PM, Guy Cox wrote:
>>> 
>>>> No, no, it IS the problem!  As Mike Ignatius explained, MP put the beads on 
>> the slide, not the coverslip, then add mountant and then the coverslip.  If you 
>> are using a #1.5 coverslip you need to put the beads directly on the 
>> coverslip.  With the beads on the slide the extra thickness of the mountant 
>> needs to be corrected for by using a thinner coverslip - #1 or #0 - which 
>> must be found by trial and error.  (But since I guess they are using very 
>> reproducible conditions they only need to do the test once).  At least it seems 
>> they have realized they have a problem.  
>>>> 
>>>>                                      Guy
>>>> 
>>>> -----Original Message-----
>>>> From: Confocal Microscopy List 
>> [mailto:[log in to unmask]] On Behalf Of MICROSCOPIA 
>> CONFOCAL y CCD
>>>> Sent: Thursday, 25 March 2010 10:15 PM
>>>> To: [log in to unmask]
>>>> Subject: Re: FocalCheck test slide
>>>> 
>>>>> Thanks a lot for your answers.
>>>> 
>>>>> Of course I put the slide upside down when using 
>>>>> inverted microscope and I asked for Molecular 
>>>>> probes about coverslip and they answered me: 
>>>>> "The coverslip thickness for the FocalCheck 
>>>>> prepared slides is 0.13-0.16 mm" It´s no ideal 
>>>>> but I think that this is not the problem.
>>>> 
>>>> M. Teresa
>>>> 
>>>> SERVICIO de MICROSCOPIA CONFOCAL y CCD
>>>> Mª Teresa Seisdedos Domínguez
>>>> Oscar Hidalgo Blanco
>>>> Amadeo Cazaña Soto
>>>> [log in to unmask]
>>>> Centro Investigaciones Biológicas (CIB) CSIC
>>>> C/Ramiro de Maeztu, 9
>>>> 28040 Madrid
>>>> Phone: + 34-91 8373112 ext.4401
>>>> Fax: + 34-91 536 04 32  
> 
> -- 
> Peter Gabriel Pitrone
> Microscope Specialist
> Max Planck Institute
> for Molecular Cell
> Biology and Genetics
> Pfotenhauerstr. 108
> 01307 Dresden
> Germany

Dr. Daniel James White BSc. (Hons.) PhD
Senior Microscopist / Image Processing and Analysis
Light Microscopy Facility
Max Planck Institute of Molecular Cell Biology and Genetics
Pfotenhauerstrasse 108
01307 DRESDEN
Germany

+49 (0)15114966933 (German Mobile)
+49  (0)351 210 2627 (Work phone at MPI-CBG)
+49  (0)351 210 1078 (Fax MPI-CBG LMF)

http://www.bioimagexd.net		BioImageXD
http://pacific.mpi-cbg.de		Fiji (is just ImageJ - batteries included)
http://www.chalkie.org.uk
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