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Hi, 
We have been very successful with DRAQ5 in both fixed and live cell imaging. It is quite resistant to photobleaching and doesn't need RNAse treatment to reduce RNA staining.
Jake Cha

> Date: Thu, 21 Apr 2011 14:51:23 -0500
> From: [log in to unmask]
> Subject: ToPro3 vs DRAQ5
> To: [log in to unmask]
> 
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> I need to buy a nuclear stain equivalent to DAPI that fluoresces in the far red.  This is for our core facility where potential users might work with mammalian tissues, plant, fungi and every other thing that has DNA in it. I want a stock that people can try out and determine whether it works for them. I would prefer only having one such dye and can't decide between TOPRO3 and DRAQ5.  Any comments on which of these (or another alternative) is the most versatile?
> 
> 
> Thomas E. Phillips, Ph.D
> Professor of Biological Sciences
> Director, Molecular Cytology Core
> 2 Tucker Hall
> University of Missouri
> Columbia, MO 65211-7400
> 573-882-4712 (office)
> 573-882-0123 (fax)
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> http://www.biology.missouri.edu/faculty/phillips.html
> http://www.biotech.missouri.edu/mcc/