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Hi,

We have used the LiveCell chamber a lot with our confocals and with MatTek
35 mm diameter Glass bottom dishes. The chambers work very well. though it
takes quite a bit of time for the humidity to reach 75%. Also we usually
used vacuum grease to seal the glass bottom dish with the chamber. I am not
certain if there would be any issues if there were no seals.

I hope this was useful.

Thanks
Abhijit Deb Roy
Biomedical Engineering Program
University of Connecticut, Storrs Campus
PhD Candidate

On Tue, Apr 5, 2011 at 7:35 PM, Leoncio Vergara <[log in to unmask]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi
>
> I was wondering if anybody could tell me about their experience with the
> Live-
> Cell stage top incubator from Pathology Devices Inc
> (http://www.pathologydevices.com/LiveCell.htm) or the Oko-lab Electric
> microscope stage incubator (http://www.oko-lab.com/39.page).
>
> Both seem to be very similar. We are looking for a solution for a BSL2
> facility
> for live cell experiments on a Olympus DSU IX81 microscope with ZDCII. We
> want to do experiments with specifically with Labtek-type chambered
> coverglasses and 35 mm petri dishes (glass bottom).
>
> I am looking for quality and reliability for temperature and environmental
> control (CO2 and Humidity) as well as easy of use. For a multiuser facility
> like
> ours, simplicity and robust design are good. For BSL2, the use of
> disposable
> chambers is also desirable, even is we have to sacrifice some in terms of
> accuracy, etc.
>
> Thanks in advance for any information
>
> Leoncio Vergara
> UTMB, Galveston Texas
>



-- 
Abhijit Deb Roy
Biomedical Engineering Program
University of Connecticut, Storrs Campus
PhD Candidate