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April 2011

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Sat, 23 Apr 2011 18:03:26 -0400
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Confocal Microscopy List <[log in to unmask]>
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Re: Live cell imaging with multiphoton confocal
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David Knecht charter <[log in to unmask]>
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*****
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Is there any disadvantage to multiphoton for cultured cells (besides cost and complexity)? Cell viability?  Phototoxicity?  Dave

On Apr 23, 2011, at 11:21 AM, Kate Luby-Phelps wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
> 
> Multiphoton has no clear advantage for cells in culture. For cell culture samples, 
> we use two photon  only to excite DAPI or other UV and near-UV fluors since we 
> had to make a choice between the Ti:S and a 405 laser on our scope. 
> 
> Kate Luby-Phelps

Dr. David Knecht    
Department of Molecular and Cell Biology
Co-head Flow Cytometry and Confocal Microscopy Facility
U-3125
91 N. Eagleville Rd.
University of Connecticut
Storrs, CT 06269
860-486-2200
860-486-4331 (fax)

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