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Hi,

I'm looking for someone having experience with confocal imaging of liver 
tissue. I'm imaging mouse liver tissue and I have some issues with 
autofluorescence in the cy3 channel coming from structure appearing like 
vesicle in hepatocytes. Those vesicles mainly show up in the Cy3 channel 
but are also visible in the FITC channel (depending on the power I use).
A spectral image with excitation at 488, shows a broad signal with a 
maximum around 580 nm.
Could someone comment on the origin of this fluorescence ? Is it possible 
that it is lipofuscine ? 

Thank you,

JP