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Other than UV dyes, native chromophores , such as NaDh, serotoni etc., are 
only accessible through multiphoton for live cell UV imaging. I can send a 
large list of our work in this area (off the list)if anyone cares to know 
about it.
Sudipta 

which are On Wed, 27 Apr 2011 18:10:16 -0600, Craig Brideau wrote
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
> 
> I second the previous lister's opinions.  At the end of the day MP 
> is really only good for thick tissue sections.  If you are looking 
> at cell layers just use a conventional 1P confocal.  The key 
> advantage of 2P is penetration depth, which is moot when you are 
> looking at cells.  That said, John's comments about shorter UV 
> imaging are still valid; it's easier to get a NIR Ti:Saph beam 
> through conventional optics than a very UV beam.  This only applies 
> if you are using dyes that need very UV excitation of course.
> 
> Craig
> 
> On Sun, Apr 24, 2011 at 6:29 AM, George McNamara
> <[log in to unmask]>wrote:
> 
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > *****
> >
> > Hi David,
> >
> > The tuning range of Ti:Sapphire (ex. 690-1040 nm) used to be problematic
> > for exciting far red and infrared fluorophores. There were some 
exceptions,
> > such as Alexa Fluor 488m 568, 594, and 633 all exciting well around 755 nm
> > (Fig 5D of Dickinson et al 2003 J Biomed Optics 8: 329-338), but since you
> > are ok with cost and complexity, adding an OPO(s) and/or multiple MP 
lasers,
> > gives you full range (see
> > http://www.coherent.com/products/?1570/Chameleon-OPO-Tuning-Range for
> > OPO).
> >
> > Depending on cell type and physiological needs, you may be able to 
suppress
> > phototoxicity by lowering O2 - cell culture at ~18% O2 is something of an
> > artifact for many cell types - by using catalase/glucose oxidase/glucose 
or
> > Oxyrase (for the latter, www.oxyrase.com, see Waterman-Storer 1993 and
> > Mikhailov 1995 Cell Motil Cytokseleton).
> >
> > Enjoy,
> >
> > George
> >
> >
> > On 4/23/2011 6:03 PM, David Knecht charter wrote:
> >
> >> *****
> >> To join, leave or search the confocal microscopy listserv, go to:
> >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> >> *****
> >>
> >> Is there any disadvantage to multiphoton for cultured cells (besides cost
> >> and complexity)? Cell viability?  Phototoxicity?  Dave
> >>
> >> On Apr 23, 2011, at 11:21 AM, Kate Luby-Phelps wrote:
> >>
> >>
> >>
> >>> *****
> >>> To join, leave or search the confocal microscopy listserv, go to:
> >>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> >>> *****
> >>>
> >>> Multiphoton has no clear advantage for cells in culture. For cell 
culture
> >>> samples,
> >>> we use two photon  only to excite DAPI or other UV and near-UV fluors
> >>> since we
> >>> had to make a choice between the Ti:S and a 405 laser on our scope.
> >>>
> >>> Kate Luby-Phelps
> >>>
> >>>
> >> Dr. David Knecht
> >> Department of Molecular and Cell Biology
> >> Co-head Flow Cytometry and Confocal Microscopy Facility
> >> U-3125
> >> 91 N. Eagleville Rd.
> >> University of Connecticut
> >> Storrs, CT 06269
> >> 860-486-2200
> >> 860-486-4331 (fax)
> >>
> >>
> >>
> >
> >
> > --
> >
> >
> > George McNamara, PhD
> > Analytical Imaging Core Facility
> > University of Miami
> >


Dr. Sudipta Maiti
Associate Professor
Dept. of Chemical Sciences
Tata Institute of Fundamental Research
Homi Bhabha Raod, Colaba, Mumbai 400005
Ph. 91-22-2278-2716 / 2539
Fax: 91-22-2280-4610
alternate e-mail: [log in to unmask]
url: biophotonics.wetpaint.com