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Hello List

We recently did an experiment where cells were stained with DAPI and Alexa 488.  These cells were grown on plastic chamber slides, then fixed and stained.  During imaging, we started seeing very weird bleaching patterns and bleedthrough of DAPI into the green viewing filter, even while just viewing the slides with a regular wide-field microscope.  We used the same staining protocols as always (mounting media with anti-quenching reagents, etc.).  The antibody that we used always worked well in our hands.  The only difference that I can think of this time is the plastic slide.  We haven't done staining on those before.  Does anybody have any similar experience with staining cells on plastic chamber slides?

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Yevgeniy Romin

Digital Microscopist
Memorial Sloan-Kettering Cancer Center
Molecular Cytology Core Facility
1275 York Ave. Box 333
New York, NY 10065
Tel.646-888-2186
Fax. 646-422-0640
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