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June 2011

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Subject:
Re: Live fixable fluorescent cell labelling
From:
"JOEL B. SHEFFIELD" <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Fri, 24 Jun 2011 12:49:33 -0400
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*****
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I just looked over the paper, and I see that we were  able to retain label
only for two days or so because, we assumed, of protein turnover in the
cells.  This may be a fundamental problem that you will have to consider.
You may want to consider a fluorescent protein label that could be
transfected in.

Joel


On Fri, Jun 24, 2011 at 12:44 PM, Michal Opas <[log in to unmask]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/**wa?A0=confocalmicroscopy<http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy>
> *****
>
> Right!
> Thanks Joel,
>
> Michal
>



-- 


Joel B. Sheffield, Ph.D
Department of Biology
Temple University
Philadelphia, PA 19122
Voice: 215 204 8839
e-mail: [log in to unmask]
URL:  http://astro.temple.edu/~jbs

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