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Leaving the discussion aside, I've seen both systems demonstrated and
recently took a look also at the Leica system. For me the main difference
(if I neglect all the licensing/patent issues) was that in the GSDIM
(Leica) they use considerably stronger lasers ( around 600 mW).

Greetings    Gabor

Light Microscopy Centre
ETH Zurich, 
Schafmattstrasse 18
CH-8093, Zurich
Switzerland

Phone: +41 44 633 6221







On 6/27/11 2:48 PM, "Mark Bates" <[log in to unmask]> wrote:

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>
>hi all,
>
>Thanks Jim for your synopsis of the related work in the EM field - I
>wasn't
>aware of that.  Just to touch on a couple of the points raised, I agree
>with
>Jim regarding the statistics of switchable molecules - this can result in
>the STORM image becoming non-linear with respect to how the intensity in
>the
>image corresponds to the local concentration of fluorophores on the
>sample. 
>This all depends on the nature of the switchable fluorophore of course,
>whether it activates once and then bleaches, or if it's reversibly
>switchable, etc.  In principle it would be very useful to treat these
>images
>as quantitative maps of fluorophore position and concentration, and this
>is
>where one needs to be careful in considering the switching statistics of
>the
>fluorophore.
>
>Regarding the name debate, PALM, STORM, FPALM etc all refer to precisely
>the
>same concept.  PALM should not be associated strictly with proteins, nor
>should STORM be associated with one type of fluorescent probe.  We made it
>clear in our original paper that the concept is applicable to any
>switchable
>fluorophore.  I don't see the need for the acronyms which have been
>subsequently coined, except where there is new science involved (such as
>GSDIM, which extends the concept to any photophysical dark state).
>
>best,
>Mark Bates, Ph.D.
>Max Planck Institute for Biophysical Chemistry
>Göttingen 37077
>Germany 
>
>--
>View this message in context:
>http://confocal-microscopy-list.588098.n2.nabble.com/Zeiss-Elyra-PALM-syst
>em-and-Nikon-N-storm-system-experiences-tp6503765p6520262.html
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