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Hi Tom,

Since a couple of years now Jakob Pernthaler concentrates bacteria using a filter approach and afterwards detects them using fluorescent markers like DAPI and even does FISH. For data acquisition he uses an upright fluorescence microscope with scanning stage. He is now based in Zuerich Switzerland www.limnology.ch - or pubmed. 

Best regards

Bruene Venus

Company affiliation: 
Carl Zeiss MicroImaging Germany
Mailto: [log in to unmask]

-------- Original-Nachricht --------
> Datum: Mon, 27 Jun 2011 14:44:51 +1000
> Von: Tom Lawson <[log in to unmask]>
> An: [log in to unmask]
> Betreff: Concentrating bacteria cells for microscope visualization.

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
> 
> Dear Confocalists,
> 
> I am having a few problems separating and concentrating bacteria cells for
> microscope visualization.
> 
> I have a 1 ml solution in an aliquot that contains some eukaryote debris
> and
> 10 to 100 bacteria cells. After spotting the centrifuge concentrate to a
> slide-well, I can see the bacteria with a nucleic stain. But the debris in
> the concentrate makes it hard to pipette and blocks seeing the bacteria.
> In
> addition, the concentrate is spread over a 6 mm slide-well which makes it
> hard to locate the bacteria.
> 
> I would be grateful for any suggestions.
> 
> Regards,
> 
> --
> Tom Lawson
> [log in to unmask]
> PhD Student,
> Macquarie University
> NSW, 2109
> Australia

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