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Cameron,

Thanks for the reply. I will be trying both ideas at my first opportunity. 
Your help is greatly appreciated, thanks again.

Kris   

On Thu, 30 Jun 2011 08:43:14 +1000, Cameron Nowell
<[log in to unmask]> wrote:
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> *****
> 
> Hi Kris,
> 
> Have you got stack buffering enabled in MetaMorph? A 2GB file on a system
> with 3GB of RAM is going to be pushing the limits a bit.
> 
> To enable stack buffering open MetaMorph, go to Edit--> Preferences and
> then go to the Stack buffering tab. You can then set the parameters as
you
> need them so save RAM, the rest will be cached on the drive until needed.
> 
> Your other option would be to open as much as you can in ImageJ (or I
> recommend Fiji) and export each slice as a an individual tiff. Then you
can
> rebuild it as a STK file in MetaMorph which will handle larger file sizes
> better. Then just repeat this for the other parts of the file (last 1000
> frames or so) you couldn't open the first time.
> 
> Cheers
> 
> Cam
> 
> 
> Cameron J. Nowell
> Microscopy Manager 
> Centre for Advanced Microscopy 
> Ludwig Institute for Cancer Research Melbourne - Parkville Branch 
> PO Box 2008 
> Royal Melbourne Hospital 
> Victoria, 3050 
> AUSTRALIA 
> Office: +61 3 9341 3155 
> Mobile: +61422882700 
> Fax: +61 3 9341 3104 
> Facility Website
> 
> 
> 
> 
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[log in to unmask]]
On
> Behalf Of krisford
> Sent: Thursday, 30 June 2011 6:39 AM
> To: [log in to unmask]
> Subject: Re: Files not opening in Metamorph?
> 
> *****
> To join, leave or search the confocal microscopy listserv, go to:
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> 
> Christian
> 
> The file is 2.076 GB in size.  Rougly 80% of the file opens in ImageJ,
and
> we are trying to adjust the file size limit to match the size of the
file. 
> So we see 4000 of the roughly 5000 frames.  ImageJ isn't letting us go
> beyond 1.6 GB, so we thought we'd go back and try Metamorph, but it
> wouldn't open at all.  We will look at the download you sent, and see how
> that goes.  If the problem persists I would definitely like to contact
you
> and send a sample .tif file.  Thanks for the assitance.
> 
> Kris
> 
> 
> On Wed, 29 Jun 2011 21:35:02 +0200, Christian Götze
> <[log in to unmask]> wrote:
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
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>> 
>> *** Commercial response ***
>> 
>> Hi Kris,
>> 
>> what does "about 2 gigabytes" mean? Is it below 2 GB or above? Although 
>> the TIFF standard uses 32 Bit pointers and thus defines TIFFs up to 4 
>> GB, several of the TIFF libraries available use "signed pointers" and 
>> therefore can't open stacks larger than 2 GB.
>> 
>> But of course the file could be simply corrupted and therefore can't be 
>> read by Metamorph.
>> 
>> Perhaps you should try to open it with other software (ImageJ,
> Photoshop).
>> 
>> In our applications we use a special modded TIFF library to open large 
>> TIFFs. You can download a trial at
>> 
>> http://www.arivis.com/en/arivis-Vision-4D
>> 
>> If your problems persist we could have a look on your data. Just write a

>> mail if interested.
>> 
>> Best,
>> Christian
>> 
>> Am 29.06.2011 21:06, schrieb krisford:
>>> *****
>>> To join, leave or search the confocal microscopy listserv, go to:
>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>>> *****
>>>
>>> Hi,
>>>
>>> I took a live streaming in Metamorph and saved it in a TIFF video file.

>>> I
>>> tried to open it later in Metamorph and got the message unable to open.
>>> The file was about 2 gigabytes and 5400 frames.  I'm using 64-Bit
> Windows
>>> and have about 3 gigs of RAM.  Can anyone help?  It would be sincerely
>>> appreciated.
>>>
>>> Thanks,
>>> Kris
> 
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