I just checked out a Chroma green fluorescent plastic slide - it seemed to fluoresce pretty well with both uv and blue LEDs so surely that should work well enough.  Doesn't have the ultra-long lifetime problem of uranyl glass.  Reproducible, robust and best of all free!  

                                   Guy

Optical Imaging Techniques in Cell Biology
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-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Martin Wessendorf
Sent: Friday, 3 June 2011 8:33 AM
To: [log in to unmask]
Subject: Re: Fluorophore excitable with 405nm and 488nm lines

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On 6/2/2011 5:16 PM, Boswell, Carl A - (cboswell) wrote:

> I just went through the effort of finding some uranyl glass to use as a fluorescent standard for a 2P imaging system.  The biggest difficulty was finding some.  After searching for and calling various commercial concerns, I managed to get a couple of pieces from scrap that the glassblowers in the Chemistry Dept. had stashed for special use.  Any idea on current sources of this material?

Other than an antique store (--uranium glass was used for its yellow or 
green color in years past), no.  We have a uranium glass slide in our 
facility but I hadn't thought about current availability--oops.

Perhaps Daniel should go with Julio's suggestions!

Martin

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