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Jewellers' supplies. Or ask a friendly jeweller for a small piece. Or
if you want a whole boule go to a manufacturer like Saint Gobain. They
gave me a very large (10cm * 2.5 cm) piece of titanium sapphire for
nothing (it was a reject, of course, but fine for such purposes). The
large emerald I played with came from someone whose research was on
gemstones - if it was natural it was worth a fortune but I assume it
wasn't!
Guy
Optical Imaging Techniques in Cell Biology
by Guy Cox CRC Press / Taylor & Francis
http://www.guycox.com/optical.htm
______________________________________________
Associate Professor Guy Cox, MA, DPhil(Oxon)
Australian Centre for Microscopy & Microanalysis,
Madsen Building F09, University of Sydney, NSW 2006
Phone +61 2 9351 3176 Fax +61 2 9351 7682
Mobile 0413 281 861
______________________________________________
http://www.guycox.net
-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]]
On Behalf Of Craig Brideau
Sent: Tuesday, 7 June 2011 3:09 AM
To: [log in to unmask]
Subject: Re: Fluorophore excitable with 405nm and 488nm lines
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That's pretty neat Guy! Where on earth would you find synthetic emerald
though?
Craig
On Sat, Jun 4, 2011 at 2:05 AM, Guy Cox <[log in to unmask]> wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hang on, the requirement was for a sample which would measure the
> RELATIVE intensities of 505 and 488nm lasers. Mild bleaching
shouldn't
> affect that. If a bit is seriously bleached, use another spot. A
quick
> scan at very low power will reveal that. You're doing pretty well if
> you can bleach an entire slide in a lifetime - and it you do, the
> replacement is free. I've never succeeded in bleaching any part but I
> have managed to laser-ablate spots on the surface! As for RI mismatch
-
> well of course there is. I'll bet there is with uranyl glass too (but
> in the opposite direction). But the killer with the U glass is that
you
> can't actually see it very well in a scanned image. If you really
want
> a solid-state unbleachable sample get a slice of emerald. I've never
> actually tried it at 405nm but it's so bright at 488 I'm sure it would
> work. Unless you have a very generous lab budget I would recommend
the
> synthetic version over the natural one!
>
> Guy
>
>
> Optical Imaging Techniques in Cell Biology
> by Guy Cox CRC Press / Taylor & Francis
> http://www.guycox.com/optical.htm
> ______________________________________________
> Associate Professor Guy Cox, MA, DPhil(Oxon)
> Australian Centre for Microscopy & Microanalysis,
> Madsen Building F09, University of Sydney, NSW 2006
>
> Phone +61 2 9351 3176 Fax +61 2 9351 7682
> Mobile 0413 281 861
> ______________________________________________
> http://www.guycox.net
>
>
> -----Original Message-----
> From: Confocal Microscopy List
[mailto:[log in to unmask]]
> On Behalf Of Cammer, Michael
> Sent: Friday, 3 June 2011 9:00 PM
> To: [log in to unmask]
> Subject: Re: Fluorophore excitable with 405nm and 488nm lines
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Depending on your tolerance, they may or may not be stable. I use
these
> all the time for aligning the microscope because they are great for
> this, although there may be an issue with refractive index mismatch
> especially with the newer TIRF objectives, but I'm not so sure as a
> reproducible sample.
> Please see
> http://www.einstein.yu.edu/aif/instructions/fluor-ref-slides/01.htm
and
>
http://www.einstein.yu.edu/aif/instructions/zeiss/liveduo/Zaxis_bleachin
> gtest/index.htm
> for two examples of bleaching.
> -Michael
>
> _________________________________________
> Michael Cammer, Assistant Research Scientist
> Skirball Institute of Biomolecular Medicine
> Lab: (212) 263-3208 Cell: (914) 309-3270
>
> ________________________________________
> From: Confocal Microscopy List [[log in to unmask]] On
> Behalf Of Guy Cox [[log in to unmask]]
> Sent: Thursday, June 02, 2011 11:32 PM
> To: [log in to unmask]
> Subject: Re: Fluorophore excitable with 405nm and 488nm lines
>
> I just checked out a Chroma green fluorescent plastic slide - it
seemed
> to fluoresce pretty well with both uv and blue LEDs so surely that
> should work well enough. Doesn't have the ultra-long lifetime problem
> of uranyl glass. Reproducible, robust and best of all free!
>
> Guy
>
> Optical Imaging Techniques in Cell Biology
> by Guy Cox CRC Press / Taylor & Francis
> http://www.guycox.com/optical.htm
> ______________________________________________
> Associate Professor Guy Cox, MA, DPhil(Oxon)
> Australian Centre for Microscopy & Microanalysis,
> Madsen Building F09, University of Sydney, NSW 2006
>
> Phone +61 2 9351 3176 Fax +61 2 9351 7682
> Mobile 0413 281 861
>
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