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*This is the FINAL announcement for the OMIBS imaging course:*



*Title:* Optical Microscopy and Imaging in the Biomedical Sciences



*When:* October 11 - October 21, 2011



*Where:* Marine Biology Laboratory, Woods Hole, MA, USA



*Tuition:* $2880 (Includes room and board, text, handouts, supplies)



*Application Deadline: *Applications accepted through June 24, 2011



*Admission application and information:*

Carol Hamel, Admissions Coordinator

Marine Biological Laboratory

7 MBL Street

Woods Hole, MA 02543-1015

(508) 289-7401

Internet: [log in to unmask]

WWW: http://www.mbl.edu (Application forms available via Adobe Acrobat)



*Course Directors: *

Robert Hard, University at Buffalo

Phone: [716] 829-3521

Email: [log in to unmask]



Colin S. Izzard, University at Albany

Phone: [518] 439-7121

Email: [log in to unmask]





*Course Description**:*



*This course will utilize the NEWLY RENOVATED and MODERNIZED FACILITIES IN
THE LOEB BLDG AT THE MARINE BIOLOGY LABORATORy.*

* *

*WE HAVE ADDED AN EXTRA DAY TO THE COURSE THIS YEAR TO *

*DEAL WITH SUPER-RESOLUTION.*

* *

*For Whom:*

Designed primarily for research scientists, postdoctoral trainees and
advanced graduate students in animal, plant, medical and material sciences.
Non-biologists seeking a comprehensive introduction to microscopy and
digital imaging will benefit greatly from this course. There are no specific
prerequisites, but an understanding of the basic principles of optics and
imaging is desirable. The course is limited to 26 students.



*Course Organization:*

The course consists of lectures, hands-on laboratory exercises, problem
sets, laboratory demonstrations and discussions that will enable the
participant to obtain and interpret microscope images of high quality, to
perform quantitative optical measurements, and to produce digital records
for documentation and analysis. In the problem sets, small groups of
students will acquire, analyze, interpret and present imaging data, thereby
demonstrating their progress toward mastering microscopy and imaging skills.



Live and fixed specimens will be provided for use in laboratory exercises
and demonstrations.



HOWEVER, STUDENTS ARE ENCOURAGED TO BRING THEIR OWN BIOLOGICAL (CELL
CULTURES, PREPARED SLIDES, ETC.) AND MATERIAL SPECIMENS TO USE THROUGHOUT
THE COURSE FOR EXERCISES, WHERE APPROPRIATE. Cell culture facilities are
available for student use. STUDENTS ALSO ARE ENCOURAGED TO MAKE APPOINTMENTS
WITH OUR EXPERIENCED COMMERCIAL FACULTY OUTSIDE OF SCHEDULED CLASSES TO
ACQUIRE DATA FROM THEIR SPECIMENS USING STATE OF THE ART EQUIPMENT
AVAILABLE. Students are highly encouraged to discuss their individual
research problems with the academic and commercial faculty.



*Topics To Be Covered Include:*



Principles of microscope design and image formation



Bright field, dark field, phase contrast, polarized light, differential
interference contrast, interference reflection, and fluorescence microscopy



Digital imaging, recording, enhancement, processing and analysis



Advanced fluorescent techniques (eg. FRAP, FLIP, speckle imaging, FRET,
FLIM, fluorescence polarization,  multi-spectral imaging, filter cubes,
fluorescent dyes)



Confocal scanning microscopy (LSM and spinning disc), multiphoton excitation
fluorescence microscopy and structured illumination systems



Image deconvolution, digital image restoration



3D image acquisition, display, and analysis



Live cell imaging



Super-resolution techniques (STED, PALM, STORM, SIM, 4PI, etc.)



Application of optical methods to live cells will be emphasized; other
specimens will be covered.



Students will have direct hands-on experience with state-of-the-art
microscopes, digital cameras, spectrophotometers, and image
acquisition/processing/analysis software provided by major optical,
electronics, and software companies. Instruction will be provided by
experienced staff from universities and industry.





*Recent faculty have included:*



Joseph A DePasquale, *Morphogenyx*

Brian Herman, *U. Texas Health Science Center*

Shinya Inoue, *Marine Biology Laboratory*

Frederick Lanni, *Carnegie Mellon University*

James McNally, *National Cancer Institute, NIH*

Butch Moomaw, *Hamamatsu*

John M. Murray,* University of Pennsylvania School of Medicine*

Rudolph Oldenberg, *Marine Biology Laboratory*

David Piston, *Vanderbilt University*

Wade Sigurdson, *University at Buffalo*

Clare M. Waterman, *National Heart Lung and Blood Institute, NIH*





*See Also:*

http://www.mbl.edu/education/courses/special_topics/om.html