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June 2011

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Subject:
Phalloidin background on transwell membrane
From:
Renato Mortara <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Tue, 14 Jun 2011 11:30:10 -0300
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Dear all
 
A colleague of mine is trying to label polarized epithelial cells grown on
transwell dishes. 
 
However, fluorescent phalloidin is labeling the dish membrane, rendering it
impossible to image the cells.
 
She tried to block with goat serum, BSA with no luck. 
 
Any suggestions ?
 
Many thanks !
 
Renato
 
Renato A. Mortara
Parasitology Division
UNIFESP - Escola Paulista de Medicina
Rua Botucatu, 862, 6th floor
São Paulo, SP
04023-062 
Brazil
Phone: 55 11 5579-8306
Fax:     55 11 5571-1095
email: [log in to unmask]
home page: www.ecb.epm.br/~ramortara

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