*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Try non fluorescent phalloidin??

>*****
>To join, leave or search the confocal microscopy listserv, go to:
>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>*****
>
>Dear all
>
>A colleague of mine is trying to label polarized epithelial cells grown on
>transwell dishes.
>
>However, fluorescent phalloidin is labeling the dish membrane, rendering it
>impossible to image the cells.
>
>She tried to block with goat serum, BSA with no luck.
>
>Any suggestions ?
>
>Many thanks !
>
>Renato
>
>Renato A. Mortara
>Parasitology Division
>UNIFESP - Escola Paulista de Medicina
>Rua Botucatu, 862, 6th floor
>São Paulo, SP
>04023-062
>Brazil
>Phone: 55 11 5579-8306
>Fax:     55 11 5571-1095
>email: [log in to unmask]
>home page: www.ecb.epm.br/~ramortara


-- 
       _      ____          __   ____   
      /  \   /          / \    /   \ \        Tobias I. Baskin
     /   /  /          /   \   \      \         Biology Department
    /_ /   __      /__ \   \       \__    611 N. Pleasant St.
   /      /          /       \   \       \        University of Massachusetts
  /      /          /         \   \       \	    Amherst, MA, 01003
/      / ___   /           \   \__/  \ ____
www.bio.umass.edu/biology/baskin
Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243