*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

What is the fluorophore?  I found that mitotracker red tends to stick to the Millipore membranes but MT green does not.  Red fluorophores tend to be more lipophilic.  
The Probes ImageiT fx might help block the sticking.

Regards,
Glen

Glen MacDonald
Core for Communication Research
Virginia Merrill Bloedel Hearing Research Center
Box 357923
University of Washington
Seattle, WA 98195-7923
(206) 616-4156

On Tue, 14 Jun 2011, Renato Mortara wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Dear all
>
> A colleague of mine is trying to label polarized epithelial cells grown on
> transwell dishes.
>
> However, fluorescent phalloidin is labeling the dish membrane, rendering it
> impossible to image the cells.
>
> She tried to block with goat serum, BSA with no luck.
>
> Any suggestions ?
>
> Many thanks !
>
> Renato
>
> Renato A. Mortara
> Parasitology Division
> UNIFESP - Escola Paulista de Medicina
> Rua Botucatu, 862, 6th floor
> São Paulo, SP
> 04023-062
> Brazil
> Phone: 55 11 5579-8306
> Fax:     55 11 5571-1095
> email: [log in to unmask]
> home page: www.ecb.epm.br/~ramortara
>