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Hi Cam,

My old institute had one, and whilst I did not directly use it the lab
working with it had a good experience. They were growing fission yeast (S.
pombe) cells and were using the combination of flow and thinning/sloping
sample chamber to fix the cells in position then alter their environment
with the perfusion system whilst monitoring rate of division. As pombe cells
are pretty much uniform in their size and shape and not adherent they got
wedged in the sample chamber at consistent positions, however I don't know
if this would be applicable for organoids.

Regards,
Graham

-- 
*Graham Wright, PhD*
Microscopy Unit Manager

Institute of Medical Biology
8A Biomedical Grove, #06-06 Immunos, Singapore 138648

O:   +65 6407 0167
E:   [log in to unmask]
W:  http://www.imb.a-star.edu.sg/imu/


On 15 June 2011 14:58, Cameron Nowell <[log in to unmask]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi List,
>
>
>
> Has anyone out there had any experience with the Onix Perfusion system?
>
>
>
> http://www.cellasic.com/ONIX.html
>
>
>
> I have a lab interested in growing organoids in gradients of various
> growth factors, cytokines etc. And they are keen to have this system. I
> wooud greatly appreciate any insight as to how it actually performs.
>
>
>
>
>
> Cheers
>
>
>
> Cam
>
>
>
>
>
>
>
> Cameron J. Nowell
> Microscopy Manager
> Centre for Advanced Microscopy
> Ludwig Institute for Cancer Research
> PO Box 2008
> Royal Melbourne Hospital
> Victoria, 3050
> AUSTRALIA
>
> Office: +61 3 9341 3155
> Mobile: +61422882700
> Fax: +61 3 9341 3104
>
> Facility Website <http://www.ludwig.edu.au/confocal/>
>
>
>
>
>
>
>
> This communication is intended only for the named recipient and may contain
> information that is confidential, legally privileged or subject to
> copyright; the Ludwig Institute for Cancer Research Ltd does not waive any
> rights if you have received this communication in error.
> The views expressed in this communication are those of the sender and do
> not necessarily reflect the views of the Ludwig Institute for Cancer
> Research Ltd.
>