*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

If you're using Alexas or Cys, check that the transwell membrane doesn't 
have a huge positive charge on it.  If so, try an overnight rinse in 
buffer with 1% BSA. That trick works to compete charge-bonded dye off of 
strongly positive (basic) protein secretion bodies in our worm wholemounts.
Julian

On 6/15/11 9:31 PM, George McNamara wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> use fluorescent anti-actin
>
>
> On 6/14/2011 10:30 AM, Renato Mortara wrote:
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>>
>> Dear all
>>
>> A colleague of mine is trying to label polarized epithelial cells 
>> grown on
>> transwell dishes.
>>
>> However, fluorescent phalloidin is labeling the dish membrane, 
>> rendering it
>> impossible to image the cells.
>>
>> She tried to block with goat serum, BSA with no luck.
>>
>> Any suggestions ?
>>
>> Many thanks !
>>
>> Renato
>>
>> Renato A. Mortara
>> Parasitology Division
>> UNIFESP - Escola Paulista de Medicina
>> Rua Botucatu, 862, 6th floor
>> São Paulo, SP
>> 04023-062
>> Brazil
>> Phone: 55 11 5579-8306
>> Fax:     55 11 5571-1095
>> email: [log in to unmask]
>> home page: www.ecb.epm.br/~ramortara
>>
>
>


-- 
Julian P.S. Smith III
Director, Winthrop Microscopy Facility
Dept. of Biology
Winthrop University
520 Cherry Rd.
Rock Hill, SC  29733

803-323-2111 x6427 (vox)
803-323-3448 (fax)
803-524-2347 (cell)
Research Website www.birdnest.org/smithj
Personal Website www.rociada-east.net