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June 2011

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From:
George McNamara <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Wed, 22 Jun 2011 06:57:53 -0400
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*****
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*****

Hi Claire,
UCSD is not in Canada, but Roger Tsien lists TagRFP-T  as a standard 
construct that can be requested at     
http://www.tsienlab.ucsd.edu/Samples/Constructs.htm    -- see 
http://www.tsienlab.ucsd.edu/Samples.htm  for instructions.


George
p.s. according to www.jcat.de  the TagRFP-T (Genbank EU582019) is not 
mammalian codon optimized (also appear the case of many other FP's whose 
sequences I've tried).

On 6/22/2011 12:22 AM, Claire Brown, Dr. wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> I have had difficulty tracking down a plasmid for TagRFP-T. I was wondering if anyone has this and would be willing to send me some? I could provide FedEx shipping information.
>
> Someone in Canada would be preferred if possible.
>
> Sincerely,
>
> Claire
>
> --------------------------------------------------------------------------------
> Claire M. Brown, PhD
> McGill University
> Life Sciences Complex Imaging Facility Director
>
>    


-- 


George McNamara, PhD
Analytical Imaging Core Facility
University of Miami

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